ROR gamma T Recombinant Rabbit Monoclonal Antibody [PSH04-38] (HA722121)
Catalog# HA722121
Rabbit Monoclonal to ROR gamma T
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WB
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IHC-P
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Human
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Rat
Lane 1: HCT 116 cell lysate
Lane 2: Rat thymus tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 55 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722121) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
ROR gamma T Recombinant Rabbit Monoclonal Antibody [PSH04-38] (HA722121)
Immunogen
Recombinant protein within human ror gamma t aa 101-300 / 497 (p51449-2).
Host
Rabbit
Positive Control
HCT 116 cell lysate, rat thymus tissue lysate, human thymus tissue, rat thymus tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH04-38
APPLICATION DILUTION
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WB
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1:2,000
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IHC-P
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1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 58 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
ROR gamma T
SYNONYMS
IMD42 antibody;MGC129539 antibody;NR1F3 antibody;Nuclear receptor ROR gamma antibody;Nuclear receptor ROR-gamma antibody;Nuclear receptor RZR gamma antibody;Nuclear receptor RZR-gamma antibody;Nuclear receptor subfamily 1 group F member 3 antibody;RAR related orphan nuclear receptor variant 2 antibody;RAR related orphan receptor C antibody;RAR related orphan receptor C, isoform a antibody;RAR related orphan receptor gamma antibody;RAR-related orphan receptor C antibody;Retinoic acid binding receptor gamma antibody;Retinoid related orphan receptor gamma antibody;Retinoid-related orphan receptor-gamma antibody;Rorc antibody;RORG antibody;RORG_HUMAN antibody;RZR GAMMA antibody;RZRG antibody;TOR antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
RAR-related orphan receptor gamma (RORγ) is a protein that in humans is encoded by the RORC (RAR-related orphan receptor C) gene. RORγ is a member of the nuclear receptor family of transcription factors. It is mainly expressed in immune cells (Th17 cells) and it also regulates circadian rhythms. It may be involved in the progression of certain types of cancer. Two isoforms are produced from the same RORC gene, probably by selection of alternative promoters. RORγ (also referred to as RORγ1) – produced from an mRNA containing exons 1 to 11. RORγt (also known as RORγ2) – produced from an mRNA identical to that of RORγ, except that the two 5'-most exons are replaced by an alternative exon, located downstream in the gene. This causes a different, shorter N-terminus.
CITATIONS
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Stat5−/− CD4+ T cells elicit anti-melanoma effect by CD4+ T cell remolding and Notch1 activation
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IHC
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Human
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Citation
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