ROR alpha Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-13] (HA751927)
Catalog# HA751927
Rabbit Monoclonal to ROR alpha
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of ROR alpha on different lysates with Rabbit anti-ROR alpha antibody (HA751927) at 1/2,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cells) cell lysate
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: Rat cerebellum tissue lysate
Lane 5: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute; ECL: K1801
Low expression: Spleen (PMID: 9226375).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751927, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 59 kDa
Observed band size: 59 kDa
Western blot analysis of ROR alpha on different lysates with Rabbit anti-ROR alpha antibody (HA751927) at 1/2,000 dilution.
Lane 1: SH-SY5Y (Human neuroblastoma cells) cell lysate
Lane 2: Mouse cerebellum tissue lysate
Lane 3: Mouse spleen tissue lysate
Lane 4: Rat cerebellum tissue lysate
Lane 5: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Exposure time: 1 minute; ECL: K1801
Low expression: Spleen (PMID: 9226375).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751927, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 59 kDa
Observed band size: 59 kDa