PRODUCT CODE: ER40122

ROCK1 Rabbit Polyclonal Antibody (ER40122)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

Western blot analysis of ROCK1 on different cell lysates using anti-ROCK1 antibody at 1/1000 dilution.<br />
Positive control:   <br />
Lane 1: NIH/3T3           <br />
Lane 2: Jurkat <br />
Lane 3: Hela
  • Western blot analysis of ROCK1 on different cell lysates using anti-ROCK1 antibody at 1/1000 dilution.<br />
Positive control:   <br />
Lane 1: NIH/3T3           <br />
Lane 2: Jurkat <br />
Lane 3: Hela
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ROCK1 antibody. Counter stained with hematoxylin.
Western blot analysis of ROCK1 on different cell lysates using anti-ROCK1 antibody at 1/1000 dilution.
Positive control:
Lane 1: NIH/3T3
Lane 2: Jurkat
Lane 3: Hela

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

ROCK1 Rabbit Polyclonal Antibody (ER40122)

Immunogen

Synthetic peptide within c-terminal residues of rock1.

Host

Rabbit

Positive Control

Hela, NIH/3T3, Jurkat, human colon cancer tissue

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

158 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • ICC

  • 1:200

  • FC

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

ROCK1

SYNONYMS

coiled-coil-containing protein kinase 1 antibody; coiled-coil-containing protein kinase I antibody; MGC131603 antibody; MGC43611 antibody; p160 Rhoassociated coiled coil-forming protein kinase antibody; p160 ROCK-1 antibody; p160 ROCK1 antibody; p160ROCK antibody; PRO0435 antibody; Renal carcinoma antigen NY REN 35 antibody; Renal carcinoma antigen NY-REN-35 antibody; Rho associated coiled coil containing protein kinase 1 antibody; Rho associated protein kinase 1 antibody; Rho kinase antibody; Rho-alpha kinase antibody; Rho-associated antibody; Rho-associated protein kinase 1 antibody; ROCK I antibody; ROCK-I antibody; ROCK1 antibody; ROCK1_HUMAN antibody; Rok antibody; rokalpha antibody

SEQUENCE SIMILARITIES

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.

TISSUE SPECIFICITY

Detected in blood platelets.

POST-TRANSLATIONAL MODIFICATION

Autophosphorylated on serine and threonine residues.; Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.

SUBCELLULAR LOCATION

Cytoplasm, cell membrane, Nucleus

FUNCTION

ROCK1 is a protein serine/threonine kinase also known as rho-associated, coiled-coil-containing protein kinase 1. ROCK1 has a ubiquitous tissue distribution, but subcellularly it is thought to colocalize with the centrosomes. This is consistent with its function as a key modulator of cell motility, tumor cell invasion, and actin cytoskeleton organization. ROCK1 has a diverse range of functions in the body. It is a key regulator of actin-myosin contraction, stability, and cell polarity. These contribute to many progresses such as regulation of morphology, gene transcription, proliferation, differentiation, apoptosis and oncogenic transformation. Other functions involve smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility. These functions are activated by phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, PFN1 and PPP1R12A.