RNA polymerase beta Recombinant Rabbit Monoclonal Antibody [JE32-42] (HA721880)
Catalog# HA721880
Rabbit Monoclonal to RNA polymerase beta
Tested for
-
WB
Reacts with
-
Escherichia coli
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of RNA polymerase beta on different lysates with Rabbit anti-RNA polymerase beta antibody (HA721880) at 1/2,000 dilution.
Lane 1: E.coli cell lysate
Lane 2: E.coli cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 150 kDa
Observed band size: 150 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721880) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: E.coli cell lysate
Lane 2: E.coli cell lysate (no heat)
Notice: no heat means the lysate is not boiled.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 150 kDa
Observed band size: 150 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721880) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.