RPL27A Rabbit Polyclonal Antibody (ER64831)
Catalog# ER64831
Rabbit Polyclonal to Ribosomal Protein L27A
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: A549 cell lysate (15 µg/Lane)
Lane 2: 293T cell lysate (15 µg/Lane)
Lane 3: HeLa cell lysate (15 µg/Lane)
Lane 4: 3T3-L1 cell lysate (15 µg/Lane)
Lane 5: Rat pancreas tissue lysate (15 µg/Lane)
Predicted band size: 17 kDa
Observed band size: 17 kDa
Exposure time: 25 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER64831) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
RPL27A Rabbit Polyclonal Antibody (ER64831)
Immunogen
Synthetic peptide within human aa 1-50.
Host
Rabbit
Positive Control
A549 cell lysate, 293T cell lysate, HeLa cell lysate, 3T3-L1 cell lysate, Rat pancreas tissue lysate, mouse pancreas tissue, mouse stomach tissue, rat pancreas tissue, rat stomach tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
APPLICATION DILUTION
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WB
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1:5,000
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IHC-P
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 17 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Ribosomal Protein L27A
SYNONYMS
60S ribosomal protein L27a antibody;FLJ43464 antibody;ribosomal protein L27a antibody;RL27A_HUMAN antibody;rpl27a antibody
FUNCTION
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L15P family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, multiple processed pseudogenes derived from this gene are dispersed through the genome.
CITATIONS
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High expression of RPL27A predicts poor prognosis in patients with hepatocellular carcinoma
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IHC
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Human
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Citation
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