Lane 1: Anti-Ribonuclease 3 Antibody, (1:500).
Lane 2: Anti-Ribonuclease 3 Antibody, (1:500), preincubated with the immunization protein.
Applications
-
WB
-
ICC
-
IHC-P
-
FC
REACTIVITY
-
Human
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
Ribonuclease 3 Mouse Monoclonal Antibody [5G4] (EM1801-17)
Immunogen
Recombinant protein within human ribonuclease 3 aa 8-140 / 160.
Host
Mouse
Positive Control
U937, Hela, SHG-44, human spleen tissue, human kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
5G4
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein G purified.
MOLECULAR WEIGHT
18 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB:1:500-1:2000
-
ICC:1:100
-
IHC-P:1:50-1:200
-
FC:1:50-1:100
TARGET
UNIPROT #
PROTEIN NAME
Ribonuclease 3
SYNONYMS
Cytotoxic ribonuclease antibody; ECP antibody; ECP_HUMAN antibody; Eosinophil cationic protein antibody; OTTHUMP00000164017 antibody; Ribonuclease 3 antibody; Ribonuclease, RNase A family, 3 antibody; RNase 3 antibody; RNASE3 antibody; RNS3 antibody
SEQUENCE SIMILARITIES
Belongs to the pancreatic ribonuclease family.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
The protein encoded by this gene belongs to the pancreatic ribonuclease family, a subset of the ribonuclease A superfamily. Cytotoxin and helminthotoxin with low-efficiency ribonuclease activity. The protein exhibits antimicrobial activity against pathogenic bacteria. Exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. Promotes E.coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content. ECP is a potent cytotoxic protein capable of killing cells of guinea pig tracheal epithelium, mammalian leukemia, epidermis carcinoma, and breast carcinoma, as well as non-mammalian cells such as parasites, bacteria, and viruses. Mature ECP is cytotoxic to human bronchial epithelial (BEAS-2B) cells by specific binding to cell surface heparan sulfate proteoglycans (HSPGs) followed by endocytosis. ECP triggers apoptosis by caspase-8 activation through mitochondria-independent pathway. Increases in chromatin condensation, sub-G1 population, PARP cleavage, and DNA fragmentation indicate that ECP induces apoptosis in human bronchial epithelial (BEAS-2B) cells.