PRODUCT CODE: ET7110-65

RhoGAP Recombinant Rabbit Monoclonal Antibody [JE53-53] (ET7110-65)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

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+
Western blot analysis of RhoGAP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SiHa cell lysate<br />
Lane 2: HepG2 cell lysate
  • Western blot analysis of RhoGAP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: SiHa cell lysate<br />
Lane 2: HepG2 cell lysate
  • Flow cytometric analysis of RhoGAP was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ET7110-65, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of RhoGAP on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET7110-65, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SiHa cell lysate
Lane 2: HepG2 cell lysate

Applications

  • WB

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

RhoGAP Recombinant Rabbit Monoclonal Antibody [JE53-53] (ET7110-65)

Immunogen

Synthetic peptide within c-terminal human rhogap.

Host

Rabbit

Positive Control

SiHa cell lysate, HepG2 cell lysate, SiHa.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JE53-53

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

172 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB:1:500-1:2,000

  • FC:1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

RhoGAP

SYNONYMS

ARHGAP 5 antibody; ARHGAP5 antibody; p190 B antibody; p190 RhoGAP antibody; Rho GTPase activating protein 5 antibody; RhoGAP 5 antibody; RhoGAP antibody; RhoGAP5 antibody

TISSUE SPECIFICITY

Detected in skin fibroblasts (at protein level).

SUBCELLULAR LOCATION

Cell membrane, cytoplasm.

FUNCTION

Rho GTPase activating protein 5 negatively regulates RHO GTPases, a family which may mediate cytoskeleton changes by stimulating the hydrolysis of bound GTP. Two transcript variants encoding different isoforms have been found for this gene. GTPase-activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of RASA1/p120GAP. Impaired autophagic degradation of lncRNA ARHGAP5-AS1 in chemoresistant cancer cells promoted chemoresistance. It can activate the transcription of ARHGAP5 in the nucleus and stimulate m6A modification of ARHGAP5 mRNA to stabilize ARHGAP5 mRNA in the cytoplasm by recruiting METTL3. Therefore, targeting ARHGAP5-AS1/ARHGAP5 axis might be a promising strategy to overcome chemoresistance in gastric cancer.