PRODUCT CODE: ET1611-3

RDC1 Recombinant Rabbit Monoclonal Antibody [SN65-09] (ET1611-3)

  • Recombinant

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of RDC1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of RDC1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of RDC1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: HUVEC cell lysate<br />
Lane 2: Jurkat cell lysate
  • Flow cytometric analysis of RDC1 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1611-3, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of RDC1 on PC-3M cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1611-3, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

RDC1 Recombinant Rabbit Monoclonal Antibody [SN65-09] (ET1611-3)

Immunogen

Synthetic peptide within c-terminal human rdc1.

Host

Rabbit

Positive Control

PC-3M cell lysates, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

SN65-09

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

41 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:1,000-1:5,000

  • FC

  • 1:10-1:100

TARGET

UNIPROT #

PROTEIN NAME

RDC1

SYNONYMS

ACKR3 antibody; atypical chemokine receptor 3 antibody; C X C chemokine receptor type 7 antibody; C-X-C chemokine receptor type 7 antibody; Chemokine (C-X-C motif) receptor 7 antibody; Chemokine C X C motif receptor 7 antibody; Chemokine orphan receptor 1 antibody; CMKOR1 antibody; CXC R7 antibody; CXC-R7 antibody; CXCR 7 antibody; CXCR-7 antibody; CXCR7 antibody; CXCR7_HUMAN antibody; G protein coupled receptor 159 antibody; G protein coupled receptor antibody; G protein coupled receptor RDC1 homolog antibody; G-protein coupled receptor 159 antibody; G-protein coupled receptor RDC1 homolog antibody; GPR159 antibody; GPRN1 antibody; RDC 1 antibody; RDC-1 antibody; RDC1 antibody

SEQUENCE SIMILARITIES

Belongs to the G-protein coupled receptor 1 family. Atypical chemokine receptor subfamily.

TISSUE SPECIFICITY

Expressed in monocytes, basophils, B-cells, umbilical vein endothelial cells (HUVEC) and B-lymphoblastoid cells. Lower expression detected in CD4+ T-lymphocytes and natural killer cells. In the brain, detected in endothelial cells and capillaries, and in mature neurons of the frontal cortex and hippocampus. Expressed in tubular formation in the kidney. Highly expressed in astroglial tumor endothelial, microglial and glioma cells. Expressed at low levels in normal CD34+ progenitor cells, but at very high levels in several myeloid malignant cell lines. Expressed in breast carcinomas but not in normal breast tissue (at protein level).

POST-TRANSLATIONAL MODIFICATION

The Ser/Thr residues in the C-terminal cytoplasmic tail may be phosphorylated.; Ubiquitinated at the Lys residues in its C-terminal cytoplasmic tail and is essential for correct trafficking from and to the cell membrane. Deubiquitinated by CXCL12-stimulation in a reversible manner.

SUBCELLULAR LOCATION

Cell membrane, Early endosome, Recycling endosome, perinuclear region.

FUNCTION

Members of the C-X-C or α chemokine family are characterized by a pair of cysteine residues separated by a single amino acid and primarily function as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell derived factor-1 (SDF-1). Receptors for the C-X-C family are G protein-coupled, seven pass transmembrane domain proteins and include proteins such as IL-8RA, IL-8RB, CXCR-3 and fusin (also designated LESTR or CXCR-4). C-X-C chemokine receptor type 7 (CXCR-7), also known as RDC-1, is a 362 amino acid receptor for SDF-1. Initially identified as a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor. CXCR-7, with CSCR-4, also acts as a coreceptor for human immunodeficiency viruses (HIV). Highly expressed in monocytes, B-cells and basophils, and highly active in various biological processes, including cell growth, cell adhesion and tumor growth, CXCR-7 may play a role in tumorigenesis.