PRODUCT CODE: ER1901-06

RBPMS Guinea Pig Polyclonal Antibody (ER1901-06)

Applications

  • WB

  • ELISA

REACTIVITY

  • Human

  • Mouse

Coating Antigens: free peptide<br />
Coating Concentration: 2 ug/ml, 100ul / well<br />
Coating Buffer: Phosphate Buffered Saline, pH7.4<br />
Secondary Antibody: Goat Anti-Guinea pig IgG (H+L), HRP Conjugated(1:1000)
  • Coating Antigens: free peptide<br />
Coating Concentration: 2 ug/ml, 100ul / well<br />
Coating Buffer: Phosphate Buffered Saline, pH7.4<br />
Secondary Antibody: Goat Anti-Guinea pig IgG (H+L), HRP Conjugated(1:1000)
Coating Antigens: free peptide
Coating Concentration: 2 ug/ml, 100ul / well
Coating Buffer: Phosphate Buffered Saline, pH7.4
Secondary Antibody: Goat Anti-Guinea pig IgG (H+L), HRP Conjugated(1:1000)

Applications

  • WB

  • ELISA

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Guinea Pig polyclonal primary

Product Name

RBPMS Guinea Pig Polyclonal Antibody (ER1901-06)

Immunogen

Synthetic peptide within human rbpms 1-30 conjugated to keyhole limpet haemocyanin.

Host

Guinea Pig

Positive Control

Ubiquitously expressed, at various levels depending on the isoform and the tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

21 kDa

Isotype

IgG

APPLICATION DILUTION

  • ELISA:1:1,000-1:5,000

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

RBPMS

SYNONYMS

RNA-binding protein with multiple splicing; Heart and RRM expressed sequence

TISSUE SPECIFICITY

Ubiquitously expressed, at various levels depending on the isoform and the tissue.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

This gene encodes a member of the RNA recognition motif family of RNA-binding proteins. The RNA recognition motif is between 80-100 amino acids in length and family members contain one to four copies of the motif. The RNA recognition motif consists of two short stretches of conserved sequence, as well as a few highly conserved hydrophobic residues. The encoded protein has a single, putative RNA recognition motif in its N-terminus. Alternative splicing results in multiple transcript variants encoding different isoforms. Acts as a coactivator of transcriptional activity. Required to increase TGFB1/Smad-mediated transactivation. Acts through SMAD2, SMAD3 and SMAD4 to increase transcriptional activity. Increases phosphorylation of SMAD2 and SMAD3 on their C-terminal SSXS motif, possibly through recruitment of TGFBR1. Promotes the nuclear accumulation of SMAD2, SMAD3 and SMAD4 proteins. Binds to poly(A) RNA.

CITATIONS

  • Scott-Hewitt, N., Perrucci,...

    Local externalization of phosphatidylserine mediates developmental synaptic pruning by microglia. The EMBO journal, 39(16), e105380.