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Rat TNF-α enzyme-linked immunoassay kit (ER0007)

Price: Regular price $499.00

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Catalog# ER0007

Assay type Sandwich ELISA

Standard curve range 1.37~1000 pg/ml

Sensitivity 0.08 pg/ml (50 μl)；0.16 pg/ml (10 μl)

Reacts with

  • Rat

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.03530.03080.0331
1.370.04820.04010.04420.0111
4.120.05690.05410.05550.0225
12.350.10480.09740.10110.0681
37.040.23520.22760.23140.1984
111.110.61210.58220.59720.5641
333.331.5681.4951.53151.4985
1000.003.4883.3703.42903.3960

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)23.299.7315.421.596.1288.4
Standard Deviation1.34.312.86.93.11.0
Coefficient of Variation(%)5.54.34.12.43.34.8

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum301.82-3.571002.94

Sensitivity

0.08 pg/ml (50 μl)；0.16 pg/ml (10 μl)

Standard curve range

1.37~1000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of human IL-6 into health rat serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 97% to 108% with an overall mean recovery of 103%.

Specifications

Product Type

ELISA Kit

Product Name

Rat TNF-α enzyme-linked immunoassay kit (ER0007)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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