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Rat IL-6 enzyme-linked immunoassay kit (ER0004)

Price: Regular price $499.00

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Catalog# ER0004

Assay type Sandwich ELISA

Standard curve range 13.72~10000 pg/ml

Sensitivity 7.05 pg/ml (50 μl)

Reacts with

  • Rat

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
00.05790.06160.0598
13.720.06530.05780.06160.0018
41.150.06880.06330.06610.0063
123.460.08530.08510.08520.0255
370.370.20240.20090.20170.1419
1111.110.57160.50640.5390.4793
3333.332.13322.10882.1212.0613
100004.56944.4844.52674.467

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)126.5792.22890.8155.2987.53062.8
Standard Deviation7.863.22427.339.587.8
Coefficient of Variation(%)6.288.44.742.9

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum30n.d.-256.898092.11

Sensitivity

7.05 pg/ml (50 μl)

Standard curve range

13.72~10000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of rat IL-6 into health rat serum sample. The un-spiked serum was used as blank in these experiments. The recovery ranged from 71% to 99% with an overall mean recovery of 89%.

Specifications

Product Type

ELISA Kit

Product Name

Rat IL-6 enzyme-linked immunoassay kit (ER0004)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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