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Rat IL-2 enzyme-linked immunoassay kit (ER0002)

Price: Regular price $499.00

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Catalog# ER0002

Assay type Sandwich ELISA

Standard curve range 10.97~8000 pg/ml

Sensitivity 8.13 pg/ml (50 μl)

Reacts with

  • Rat

Safety datasheet

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
00.06450.06360.0641
10.970.07310.07330.07320.0092
32.920.08720.08730.08730.0232
98.770.13820.14330.14080.0767
296.30.28120.29530.28830.2242
888.890.64220.65430.64830.5842
2666.671.54321.51531.52931.4652
80003.08813.19323.14073.0766

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)139708.72461.3141.5712.22421.2
Standard Deviation7.521.9120.79.334.2130.7
Coefficient of Variation(%)5.43.14.96.64.85.4

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum309.9-387.75092.4

Sensitivity

8.13 pg/ml (50 μl)

Standard curve range

10.97~8000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of rat IL-2 into health rat serum sample. The un-spiked serum was used as blank in these experiments. The recovery ranged from 83% to 110% with an overall mean recovery of 95%.

Specifications

Product Type

ELISA Kit

Product Name

Rat IL-2 enzyme-linked immunoassay kit (ER0002)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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