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Rat CRP/C-Reactive Protein enzyme-linked immunoassay kit (ER0009)

Price: Regular price $499.00

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Catalog# ER0009

Assay type Sandwich ELISA

Standard curve range 81.92~20000 pg/ml

Sensitivity 27.24 pg/ml (50 μl)；63.91 pg/ml (10 μl)

Reacts with

  • Rat

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PERFORMANCE CHARACTERISTICS

Product detail

pg/ml O.D. Average Corrected
0.000.02480.02570.0253
81.920.03920.03710.03820.0232
204.800.07770.07920.07850.0635
512.000.15530.13990.14760.1326
1280.000.35100.35450.35280.3378
3200.000.90280.87890.89090.8759
8000.002.04102.12802.08452.0695
20000.003.64603.92103.78353.7685

Precision

Intra-assay PrecisionInter-assay Precision
Sample Number S1S2S3S1S2S3
222222666
Average(pg/ml)486.62033.56246.1483.82222.26448.2
Standard Deviation25.5121.9330.315.4112.6123.97
Coefficient of Variation(%)5.26.04.83.25.11.9

Sample Values

Sample MatrixSample EvaluatedRange (pg/ml)Detectable (%) Mean of Detectable (pg/ml)
Serum3096.31-1708.35100398.28

Sensitivity

27.24 pg/ml (50 μl)；63.91 pg/ml (10 μl)

Standard curve range

81.92~20000 pg/ml

Recovery

The spike recovery was evaluated by spiking 3 levels of rat CRP into health rat serum sample. The un-spiked serum was used as blank in this experiment. The recovery ranged from 74% to 137% with an overall mean recovery of 103%.

Specifications

Product Type

ELISA Kit

Product Name

Rat CRP/C-Reactive Protein enzyme-linked immunoassay kit (ER0009)

Detection method

Colorimetric

Sample type

Serum

Assay type

Sandwich ELISA

Category

Fully Ready-to-Use

PROPERTIES

Storage instructions

Store at +4℃

Kits Components

Coated Plate

96 well

Standard

9 vial

Detect Antibody

6 ml/bottle

Streptavidin-HRP

12 ml/bottle

Assay Buffer

12 ml/bottle

Stop Solution

12 ml/bottle

Wash Buffer

50 ml/bottle

Sealer Film

6 pieces

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