Applications
-
WB
-
ICC
-
IF
-
IHC-P
REACTIVITY
-
Human
-
Mouse
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
RANTES Recombinant Rabbit Monoclonal Antibody [JM03-45] (ET1705-70)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Mouse thymus tissue lysate, Hela, HUVEC, human tonsil tissue, human breast cancer tissue, human lung caner tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM03-45
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
10 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:500-1:1,000
-
ICC/IF
-
1:50-1:200
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
RANTES
SYNONYMS
Beta chemokine RANTES antibody; Beta chemokine RANTES precursor antibody; C C motif chemokine 5 antibody; CCL 5 antibody; CCL5 antibody; CCL5_HUMAN antibody; Chemokine (C C motif) ligand 5 antibody; Chemokine CC Motif Ligand 5 antibody; D17S136E antibody; EoCP antibody; Eosinophil chemotactic cytokine antibody; MGC17164 antibody; RANTES(4-68) antibody; Regulated upon activation normally T expressed and presumably secreted antibody; SCYA 5 antibody; SCYA5 antibody; SIS delta antibody; SIS-delta antibody; SISd antibody; Small inducible cytokine A5 (RANTES) antibody; Small inducible cytokine A5 antibody; Small inducible cytokine subfamily A (Cys Cys) member 5 antibody; Small-inducible cytokine A5 antibody; T cell specific protein p288 antibody; T cell specific RANTES protein antibody; T cell-specific protein P228 antibody; T-cell-specific protein RANTES antibody; TCP 228 antibody; TCP228 antibody
SEQUENCE SIMILARITIES
Belongs to the intercrine beta (chemokine CC) family.
TISSUE SPECIFICITY
Expressed in the follicular fluid (at protein level). T-cell and macrophage specific.
POST-TRANSLATIONAL MODIFICATION
N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.; The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.