Raf1 Recombinant Rabbit Monoclonal Antibody [JJ086-06] (ET1701-21)
Catalog# ET1701-21
Rabbit Monoclonal to Raf1
-
WB
-
Human
-
Mouse
-
Rat
Lane 1: A375 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: RAW264.7 cell lysate
Lane 4: NIH/3T3 cell lysate
Lane 5: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 73 kDa
Observed band size: 73 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1701-21) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Raf1 Recombinant Rabbit Monoclonal Antibody [JJ086-06] (ET1701-21)
Immunogen
Synthetic peptide within human raf1 aa 1-50/648.
Host
Rabbit
Positive Control
A375 cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, A549 cell lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JJ086-06
RRID
APPLICATION DILUTION
-
WB
-
1:1,000-1:2,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 73 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Raf1
SYNONYMS
c Raf antibody;C-raf antibody;C-Raf proto-oncogene, serine/threonine kinase antibody;CMD1NN antibody;Craf 1 transforming gene antibody;cRaf antibody;Craf1 transforming gene antibody;EC 2.7.11.1 antibody;kinase Raf1 antibody;Murine sarcoma 3611 oncogene 1 antibody;NS5 antibody;Oncogene MIL antibody;Oncogene RAF1 antibody;OTTHUMP00000160218 antibody;OTTHUMP00000207813 antibody;OTTHUMP00000209389 antibody;Protein kinase raf 1 antibody;Proto-oncogene c-RAF antibody;Raf 1 antibody;Raf 1 proto oncogene serine/threonine kinase antibody;RAF antibody;Raf proto oncogene serine/threonine protein kinase antibody;RAF proto-oncogene serine/threonine-protein kinase antibody;RAF-1 antibody;RAF1 antibody;RAF1_HUMAN antibody;Similar to murine leukemia viral (V-raf-1) oncogene homolog 1 antibody;TRANSFORMING REPLICATION-DEFECTIVE MURINE RETROVIRUS 3611-MSV antibody;v raf 1 murine leukemia viral oncogene homolog 1 antibody;v-raf murine sarcoma viral oncogene homolog 1 antibody;v-raf-1 murine leukemia viral oncogene-like protein 1 antibody;vraf1 murine leukemia viral oncogene homolog 1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
TISSUE SPECIFICITY
In skeletal muscle, isoform 1 is more abundant than isoform 2.
POST-TRANSLATIONAL MODIFICATION
Phosphorylation at Thr-269, Ser-338, Tyr-341, Thr-491 and Ser-494 results in its activation. Phosphorylation at Ser-29, Ser-43, Ser-289, Ser-296, Ser-301 and Ser-642 by MAPK1/ERK2 results in its inactivation. Phosphorylation at Ser-259 induces the interaction with YWHAZ and inactivates kinase activity. Dephosphorylation of Ser-259 by the complex containing protein phosphatase 1, SHOC2 and M-Ras/MRAS relieves inactivation, leading to stimulate RAF1 activity. Phosphorylation at Ser-338 by PAK1 and PAK5 and Ser-339 by PAK1 is required for its mitochondrial localization. Phosphorylation at Ser-621 in response to growth factor treatment stabilizes the protein, possibly by preventing proteasomal degradation. Phosphorylation at Ser-289, Ser-296, Ser-301, Ser-338 and Ser-621 are somehow linked to the methylation potential of cells. Treatment of cells with HGF in the presence of the methylation inhibitor 5'-methylthioadenosine (MTA) results in increased phosphorylation at Ser-338 and Ser-621 and decreased phosphorylation at Ser-296, Ser-301 and Ser-338. Dephosphorylation at Ser-338 by PPP5C results in an activity decrease.; Methylated at Arg-563 in response to EGF treatment. This modification leads to destabilization of the protein, possibly through proteasomal degradation.
SUBCELLULAR LOCATION
Cytoplasm, Cell membrane, Mitochondrion, Nucleus.
FUNCTION
RAF proto-oncogene serine/threonine-protein kinase, also known as proto-oncogene c-RAF or simply c-Raf or even Raf-1, is an enzyme that in humans is encoded by the RAF1 gene. The c-Raf protein is part of the ERK1/2 pathway as a MAP kinase (MAP3K) that functions downstream of the Ras subfamily of membrane associated GTPases. C-Raf is a member of the Raf kinase family of serine/threonine-specific protein kinases, from the TKL (Tyrosine-kinase-like) group of kinases. Raf-1 is activated in response to activation of a variety of tyrosine kinase receptors as well as in response to pp60v-Src expression.
