PRODUCT CODE: ER1802-61

Rae1 Rabbit Polyclonal Antibody (ER1802-61)

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Rae1 on different lysates using anti-Rae1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3<br />
   Lane 2: Mouse testis tissue<br />
 Lane 3: Rat testis tissue
  • Western blot analysis of Rae1 on different lysates using anti-Rae1 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3<br />
   Lane 2: Mouse testis tissue<br />
 Lane 3: Rat testis tissue
  • ICC staining Rae1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Rae1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining Rae1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.Flow cytometric analysis of LOVO cells with Rae1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Western blot analysis of Rae1 on different lysates using anti-Rae1 antibody at 1/500 dilution.
Positive control:
Lane 1: PC-3
Lane 2: Mouse testis tissue
Lane 3: Rat testis tissue

Applications

  • WB

  • ICC

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Rae1 Rabbit Polyclonal Antibody (ER1802-61)

Immunogen

Synthetic peptide within human rae1 aa 319-368 / 368.

Host

Rabbit

Positive Control

PC-3, mouse testis tissue, rat testis tissue, LOVO, MCF-7, PC-3M.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

41 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1,000

  • ICC

  • 1:50-1:200

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Rae1

SYNONYMS

dJ481F12.3 antibody; dJ800J21.1 antibody; FLJ30608 antibody; Homolog of yeast Rae1 (Bharathi) mRNA associated protein of 41 kDa (Kraemer) antibody; Homolog of yeast Rae1 mRNA associated protein of 41 kDa antibody; MGC117333 antibody; MGC126076 antibody; MGC126077 antibody; MIG 14 antibody; MIG14 antibody; Migration inducing gene 14 antibody; Mnrp 41 antibody; Mnrp41 antibody; mRNA associated protein mrnp 41 antibody; mRNA binding protein 41 kD antibody; mRNA export factor antibody; mRNA export protein antibody; mRNA-associated protein mrnp 41 antibody; MRNP 41 antibody; MRNP41 antibody; RAE 1 antibody; RAE1 (RNA export 1 S.pombe) homolog antibody; rae1 antibody; RAE1 homolog antibody; Rae1 protein homolog antibody; RAE1 RNA export 1 homolog (S. pombe) antibody; RAE1 RNA export 1 homolog antibody; RAE1L_HUMAN antibody; RNA export 1 antibody; RNA export 1 homolog antibody

SEQUENCE SIMILARITIES

Belongs to the WD repeat rae1 family.

SUBCELLULAR LOCATION

Nucleus. Cytoskeleton.Cytoplasm.

FUNCTION

Mrnp41 (mRNA-binding protein, 41-KD), also known as Rae1 protein homolog and mRNA export factor, is a 368 amino acid protein that binds mRNA and is involved in nucleocytoplasmic transport. Though characterized in both the nucleus and cytoplasm, mrnp41 is primarily localized to the nuclear pore complex in the nuclear envelope. Mutations in mrnp41 may result in the accumulation of poly(A)-containing mRNA in the nucleus, further supporting the role of mrnp41 as a primary nuclear exporter of mRNA. Along with Nup98, mrnp41 has been shown to regulate E-cadherin, an activating subunit of the anaphase-promoting complex complex, which results in the prevention of securin degradation, therefore suggesting that mrnp41 may play a potential role in maintaining euploidy. Also, during mitosis, both mrnp41 and NuMA have been shown to be required for bipolar spindle formation.