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PRODUCT CODE: HA500010

Rad51B Rabbit Polyclonal Antibody (HA500010)

Applications

  • WB

REACTIVITY

  • Human

  • Rat

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Western blot analysis of Rad51B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500010, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: SKBR3 cell lysate<br /> Lane 2: PC-12 cell lysate
  • Western blot analysis of Rad51B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500010, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br /> Positive control: <br /> Lane 1: SKBR3 cell lysate<br /> Lane 2: PC-12 cell lysate
Western blot analysis of Rad51B on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA500010, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SKBR3 cell lysate
Lane 2: PC-12 cell lysate

Applications

  • WB

REACTIVITY

  • Human

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Rad51B Rabbit Polyclonal Antibody (HA500010)

Immunogen

Recombinant protein within c-terminal human rad51b.

Host

Rabbit

Positive Control

SKBR3 cell lysate, PC-12 cell lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

42 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

O15315

PROTEIN NAME

Rad51B

SYNONYMS

DNA repair protein RAD51 homolog 2 antibody; hREC2 antibody; MGC34245 antibody; OTTHUMP00000212251 antibody; OTTHUMP00000212253 antibody; OTTHUMP00000212254 antibody; OTTHUMP00000212255 antibody; R51H2 antibody; RA51B_HUMAN antibody; RAD51 homolog B (S. cerevisiae) antibody; RAD51 homolog B antibody; RAD51 like 1 antibody; RAD51 like protein 1 antibody; RAD51-like protein 1 antibody; Rad51B antibody; RAD51L1 antibody; REC2 antibody; RecA like protein antibody; Recombination repair protein antibody

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. Binds single-stranded DNA and double-stranded DNA and has DNA-dependent ATPase activity. Part of the RAD21 paralog protein complex BCDX2 which acts in the BRCA1-BRCA2-dependent HR pathway. Upon DNA damage, BCDX2 acts downstream of BRCA2 recruitment and upstream of RAD51 recruitment. BCDX2 binds predominantly to the intersection of the four duplex arms of the Holliday junction and to junction of replication forks. The BCDX2 complex was originally reported to bind single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. The BCDX2 subcomplex RAD51B:RAD51C exhibits single-stranded DNA-dependent ATPase activity suggesting an involvement in early stages of the HR pathway.