PRODUCT CODE: ET1705-96

Rad51 Recombinant Rabbit Monoclonal Antibody [JM54-26] (ET1705-96)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Rad51 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse testis tissue lysate<br />
Lane 2: Jurkat cell lysate
  • Western blot analysis of Rad51 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Mouse testis tissue lysate<br />
Lane 2: Jurkat cell lysate
  • ICC staining of Rad51 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-96, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Rad51 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-96, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Rad51 in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-96, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Rad51 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-96, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Rad51 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-96, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Rad51 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1705-96, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of Rad51 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ET1705-96, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of Rad51 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-96, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse testis tissue lysate
Lane 2: Jurkat cell lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

  • IP

  • FC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Rad51 Recombinant Rabbit Monoclonal Antibody [JM54-26] (ET1705-96)

Immunogen

Synthetic peptide within human rad51 aa 1-50 / 339.

Host

Rabbit

Positive Control

Mouse testis tissue lysates, Hela, LOVO, PANC-1, rat epididymis tissue, human placenta tissue, mouse testis tissue, Jurkat.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM54-26

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

37 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

  • IP

  • 1:10-1:50 FC

TARGET

UNIPROT #

PROTEIN NAME

Rad51

SYNONYMS

BRCA1/BRCA2 containing complex, subunit 5 antibody; BRCC 5 antibody; BRCC5 antibody; DNA repair protein RAD51 homolog 1 antibody; DNA repair protein rhp51 antibody; FANCR antibody; hRAD51 antibody; HsRAD51 antibody; HsT16930 antibody; MRMV2 antibody; Rad 51 antibody; RAD51 antibody; RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) antibody; RAD51 homolog A antibody; RAD51 homolog antibody; RAD51 recombinase antibody; RAD51, S. cerevisiae, homolog of antibody; RAD51_HUMAN antibody; RAD51A antibody; RECA antibody; RecA like protein antibody; RecA, E. coli, homolog of antibody; Recombination protein A antibody

SEQUENCE SIMILARITIES

Belongs to the RecA family. RAD51 subfamily.

TISSUE SPECIFICITY

Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.

POST-TRANSLATIONAL MODIFICATION

Ubiquitinated by the SCF(FBH1) E3 ubiquitin ligase complex, regulating RAD51 subcellular location and preventing its association with DNA. Ubiquitinated by RFWD3 in response to DNA damage: ubiquitination leads to degradation by the proteasome, promoting homologous recombination.; Phosphorylated. Phosphorylation of Thr-309 by CHEK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination. Phosphorylation by ABL1 inhibits function.

SUBCELLULAR LOCATION

Mitochondrion matrix, Nucleus, centrosome, Cytoplasm, perinuclear region, Chromosome.

FUNCTION

Rad51 (RECA, BRCC5) interacts with BRCA1 and BRCA2 to influence subcellular localization and cellular response to DNA damage. BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis from deregulation of Rad51. Rad52 forms a heptameric ring that binds single-stranded DNA ends and catalyzes DNA-DNA interaction necessary for the annealing of complementary strands. Rad52 can interact with Rad51. Rad54A of the DEAD-like helicase superfamily binds to double-strand DNA and induces a DNA topological change, which is thought to facilitate homologous DNA pairing and stimulate DNA recombination. Rad54B of the DEAD-like helicase superfamily binds to double-stranded DNA and displays ATPase activity in the presence of DNA. Rad54B is abundant in testis and spleen, and mutations of this gene occur in primary lymphoma and colon cancer. MRE11 (meiotic recombination 11, ATLD, HNGS1) is a nuclear 3′-5′ exonuclease/endonuclease that associates with Rad50 and influences homologous recombination, telomere length maintenance, and DNA double-strand break repair. MRE11 is most abundant in proliferating tissues.

CITATIONS

  • Zhao, M., Wang, Y., Jia,......

    Zhao, M., Wang, Y., Jia, X., Liu, W., Zhang, X., & Cui, J. (2021). The effect of ochratoxin A on cytotoxicity and glucose metabolism in human esophageal epithelium Het-1A cells. Toxicon : official journal of the International Society on Toxinology, 198, 80–92.