Western blot analysis of Rad21 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1803-73, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Lane 1: Mouse ovary tissue lysate
Lane 2: Daudi cell lysate
Rabbit polyclonal primary
Rad21 Rabbit Polyclonal Antibody (ER1803-73)
Recombinant protein within human rad21 aa 252-456 / 631.
Mouse ovary tissue lysate, Daudi cell lysate, MG-63, SiHa, SK-Br-3, rat brain tissue, human thyroid gland carcinoma tissue, human colon tissue, mouse testis tissue.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
CDLS4 antibody;Double-strand-break repair protein rad21 homolog antibody;hHR21 antibody;HR21 antibody;HRAD21 antibody;KIAA0078 antibody;MCD1 antibody;Nuclear matrix protein 1 antibody;NXP-1 antibody;NXP1 antibody;Protein involved in DNA double-strand break repair antibody;RAD21 antibody;RAD21 homolog (S. pombe) antibody;RAD21 homolog antibody;RAD21_HUMAN antibody;Scc1 antibody;SCC1 homolog antibody
Belongs to the rad21 family.
Expressed in the gut (at protein level).
Regulated in a cell cycle-dependent manner: expression increases in late S phase and reaches maximum in G2 at the nucleotide level. Not regulated during the cell cycle (at protein level).
Cleaved by separase/ESPL1 at the onset of anaphase; this cleavage is required for sister chromatid separation and cytokinesis. Cleaved by caspase-3/CASP3 or caspase-7/CASP7 at the beginning of apoptosis.; Phosphorylated; becomes hyperphosphorylated in M phase of cell cycle. The large dissociation of cohesin from chromosome arms during prophase may be partly due to its phosphorylation by PLK1.
Centromere, Chromosome, Nucleus.
Cleavable component of the cohesin complex, involved in chromosome cohesion during cell cycle, in DNA repair, and in apoptosis. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At metaphase-anaphase transition, this protein is cleaved by separase/ESPL1 and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis. Also plays a role in apoptosis, via its cleavage by caspase-3/CASP3 or caspase-7/CASP7 during early steps of apoptosis: the C-terminal 64 kDa cleavage product may act as a nuclear signal to initiate cytoplasmic events involved in the apoptotic pathway.
Just like the interactions between antigens and antibodies, the higher the affinity between you and us the better.