RAC1 Recombinant Rabbit Monoclonal Antibody [PSH05-09] (HA722225)
Catalog# HA722225
Rabbit Monoclonal to RAC1
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WB
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IF-Cell
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Human
Lane 1: HEK-293 cell lysate
Lane 2: Huh7 cell lysate
Lane 3: HUVEC cell lysate
Lane 4: HL-60 cell lysate
Lane 5: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722225) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
RAC1 Recombinant Rabbit Monoclonal Antibody [PSH05-09] (HA722225)
Immunogen
Recombinant protein within human rac1 aa 1-192 / 192.
Host
Rabbit
Positive Control
HEK-293 cell lysate, Huh7 cell lysate, HUVEC cell lysate, HL-60 cell lysate, HeLa cell lysate, HL-60.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH05-09
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:100
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 21 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
RAC1
SYNONYMS
Cell migration inducing gene 5 protein antibody;Cell migration-inducing gene 5 protein antibody;MGC111543 antibody;MIG5 antibody;Migration inducing gene 5 antibody;Migration inducing protein 5 antibody;p21 Rac1 antibody;p21-Rac1 antibody;Rac 1 antibody;RAC1 antibody;RAC1_HUMAN antibody;Ras like protein TC25 antibody;Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody;Ras-like protein TC25 antibody;Ras-related C3 botulinum toxin substrate 1 antibody;Rho family small GTP binding protein Rac1 antibody;TC 25 antibody;TC25 antibody
SUBCELLULAR LOCATION
Cell membrane, Cell projection, Cytoplasm, Membrane, Nucleus, Synapse.
FUNCTION
Rac1, also known as Ras-related C3 botulinum toxin substrate 1, is a protein found in human cells. It is encoded by the RAC1 gene. This gene can produce a variety of alternatively spliced versions of the Rac1 protein, which appear to carry out different functions. Rac1 is a small (~21 kDa) signalling G protein (more specifically a GTPase), and is a member of the Rac subfamily of the family Rho family of GTPases. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of GLUT4 translocation to glucose uptake, cell growth, cytoskeletal reorganization, antimicrobial cytotoxicity, and the activation of protein kinases. Rac1 is a pleiotropic regulator of many cellular processes, including the cell cycle, cell-cell adhesion, motility (through the actin network), and of epithelial differentiation (proposed to be necessary for maintaining epidermal stem cells).
CITATIONS
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Yasi Deng,et al
Effective extraction of Xuetongsu and its role in preventing RA synovial hyperplasia by targeting synovial cell migration and apoptosis
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WB
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Mouse
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Citation
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