PRODUCT CODE: ET1703-80

Rac1-2-3 Recombinant Rabbit Monoclonal Antibody [JM11-29] (ET1703-80)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Rac1-2-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: PC-12 cell lysate
  • Western blot analysis of Rac1-2-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: MCF-7 cell lysate<br />
Lane 2: PC-12 cell lysate
  • ICC staining of Rac1-2-3 in 293T cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Rac1-2-3 in Hela cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • ICC staining of Rac1-2-3 in NIH/3T3 cells (red). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1703-80, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®594 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Rac1-2-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-80, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Rac1-2-3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1703-80, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis of Rac1-2-3 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1703-80, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: MCF-7 cell lysate
Lane 2: PC-12 cell lysate

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Rac1-2-3 Recombinant Rabbit Monoclonal Antibody [JM11-29] (ET1703-80)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

MCF-7 cell lysate, PC-12 cell lysate, 293T, Hela, NIH/3T3, human tonsil tissue, human spleen tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM11-29

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

MOLECULAR WEIGHT

21/24 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Rac1-2-3

SYNONYMS

Cell migration inducing gene 5 protein antibody; EN 7 antibody; OTTMUSP00000004488 antibody; p21 Rac3 antibody; p21-Rac3 antibody; Rac1B antibody; RAC3 antibody; RAC3_HUMAN antibody; RAS related C3 botulinum substrate 3 antibody; Ras related C3 botulinum toxin substrate 3 (rho family small GTP binding protein Rac3) antibody; Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac3) antibody; Ras-related C3 botulinum toxin substrate 3 antibody; Rho family small GTP binding protein Rac3 antibody; RP23-84C12.18 antibody; EN-7 antibody; EN7 antibody; GX antibody; HSPC 022 antibody; HSPC022 antibody; p21 Rac 2 antibody; p21 Rac2 antibody; p21-Rac2 antibody; p21Rac2 antibody; RAC 2 antibody; Rac2 antibody; RAC2_HUMAN antibody; Ras related C3 botulinum toxin substrate 2 (rho family, small GTP binding protein Rac2 antibody; Ras related C3 botulinum toxin substrate 2 antibody; Ras related C3 botulinum toxin substrate 3 (rho family, small GTP binding protein Rac2) antibody; Ras related C3 botulinum toxin substrate 3 antibody; Ras-related C3 botulinum toxin substrate 2 antibody; Rho family small GTP binding protein Rac 2 antibody; Rho family small GTP binding protein Rac2 antibody; Small G protein antibody; Cell migration-inducing gene 5 protein antibody; MGC111543 antibody; MIG5 antibody; Migration inducing gene 5 antibody; Migration inducing protein 5 antibody; p21 Rac1 antibody; p21-Rac1 antibody; Rac 1 antibody; RAC1 antibody; RAC1_HUMAN antibody; Ras like protein TC25 antibody; Ras related C3 botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1) antibody; Ras-like protein TC25 antibody; Ras-related C3 botulinum toxin substrate 1 antibody; Rho family small GTP binding protein Rac1 antibody; TC 25 antibody; TC25 antibody

SEQUENCE SIMILARITIES

Belongs to the small GTPase superfamily. Rho family.

TISSUE SPECIFICITY

Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.

POST-TRANSLATIONAL MODIFICATION

(Microbial infection) AMPylation at Tyr-32 and Thr-35 are mediated by bacterial enzymes in case of infection by H.somnus and V.parahaemolyticus, respectively. AMPylation occurs in the effector region and leads to inactivation of the GTPase activity by preventing the interaction with downstream effectors, thereby inhibiting actin assembly in infected cells. It is unclear whether some human enzyme mediates AMPylation; FICD has such ability in vitro but additional experiments remain to be done to confirm results in vivo.; GTP-bound active form is ubiquitinated by HACE1, leading to its degradation by the proteasome.; (Microbial infection) Glycosylated at Tyr-32 by Photorhabdus asymbiotica toxin PAU_02230. Mono-O-GlcNAcylation by PAU_02230 inhibits downstream signaling by an impaired interaction with diverse regulator and effector proteins of Rac and leads to actin disassembly.

SUBCELLULAR LOCATION

Cell membrane. Cytoplasm.

FUNCTION

Plasma membrane-associated small GTPase which cycles between active GTP-bound and inactive GDP-bound states. In its active state, binds to a variety of effector proteins to regulate cellular responses such as secretory processes, phagocytosis of apoptotic cells, epithelial cell polarization and growth-factor induced formation of membrane ruffles. Rac1 p21/rho GDI heterodimer is the active component of the cytosolic factor sigma 1, which is involved in stimulation of the NADPH oxidase activity in macrophages (By similarity). Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. Isoform B has an accelerated GEF-independent GDP/GTP exchange and an impaired GTP hydrolysis, which is restored partially by GTPase-activating proteins. It is able to bind to the GTPase-binding domain of PAK but not full-length PAK in a GTP-dependent manner, suggesting that the insertion does not completely abolish effector interaction.