RAB7 Rabbit Polyclonal Antibody (ER1802-53)
Catalog# ER1802-53
Rabbit Polyclonal to RAB7
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: MCF-7 cell lysate (20 µg/Lane)
Lane 2: Rat brain tissue lysate (20 µg/Lane)
Lane 2: Human brain tissue lysate (20 µg/Lane)
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 2 minutes;
15% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1802-53) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
RAB7 Rabbit Polyclonal Antibody (ER1802-53)
Immunogen
Recombinant protein within human rab7 aa 43-200 / 207.
Host
Rabbit
Positive Control
MCF-7, rat brain tissue, human brain tissue, human tonsil tissue, human liver tissue, human colon cancer tissue, mouse colon tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 23 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
RAB7
SYNONYMS
CMT2B antibody;PRO2706 antibody;PSN antibody;RAB7, member RAS oncogene family antibody;RAB7A antibody;RAB7A, member RAS oncogene family antibody;RAB7A_HUMAN antibody;Ras associated protein RAB7 antibody;Ras related protein Rab7 antibody;Ras related protein Rab7a antibody;Ras-related protein Rab-7a antibody
SEQUENCE SIMILARITIES
Belongs to the small GTPase superfamily. Rab family.
TISSUE SPECIFICITY
Widely expressed; high expression found in skeletal muscle.
SUBCELLULAR LOCATION
Endosome. Lysosome.
FUNCTION
The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral/Rec, Rap, R-Ras, and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.
CITATIONS
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Tian Wenjun,et al
Calmodulin-like 5 promotes PEDV replication by regulating late-endosome synthesis and innate immune response
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WB
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Green Monkey
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Citation
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