RAB27A Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH24-10] (HA751925)
Catalog# HA751925
Rabbit Monoclonal to RAB27A
Tested for
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WB
-
IHC-P
-
FC
-
IP
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of RAB27A on different lysates with Rabbit anti-RAB27A antibody (HA751925) at 1/5,000 dilution.
Lane 1: SK-MEL-28 (Human malignant melanoma cells) cell lysate
Lane 2: Raji (Human Burkitt's lymphoma cells) cell lysate
Lane 3: HL-60 (Human acute promyelocytic leukemia cells) cell lysate
Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 5: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lane 6: U-87 MG (Human glioblastoma cells) cell lysate
Lane 7: NCI-H226 (Human pleural mesothelioma cells) cell lysate
Lane 8: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Negative control : Raji (PMID: 15548590).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751925, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 25 kDa
Observed band size: 27 kDa
Western blot analysis of RAB27A on different lysates with Rabbit anti-RAB27A antibody (HA751925) at 1/5,000 dilution.
Lane 1: SK-MEL-28 (Human malignant melanoma cells) cell lysate
Lane 2: Raji (Human Burkitt's lymphoma cells) cell lysate
Lane 3: HL-60 (Human acute promyelocytic leukemia cells) cell lysate
Lane 4: Jurkat (Human T-lymphoblastic cells) cell lysate
Lane 5: K-562 (Human chronic myelogenous leukemia cells) cell lysate
Lane 6: U-87 MG (Human glioblastoma cells) cell lysate
Lane 7: NCI-H226 (Human pleural mesothelioma cells) cell lysate
Lane 8: PC-12 (Rat pheochromocytoma cells (undifferentiated)) cell lysate
Lysates/proteins at 15 µg/Lane.
Exposure time: 10 seconds; ECL: K1801
Negative control : Raji (PMID: 15548590).
Blocking: 5% NFDM/TBST, 1 hour at room temperature
Primary antibody: HA751925, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 °C
Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature
Predicted band size: 25 kDa
Observed band size: 27 kDa