Rab1A Recombinant Rabbit Monoclonal Antibody [PSH03-62] (HA722021)
Catalog# HA722021
Rabbit Monoclonal to Rab1A
Tested for
-
WB
-
IHC-P
-
IF-Cell
-
FC
Reacts with
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Human
-
Mouse
-
Rat
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
☑ Relative expression (RE)
Western blot analysis of Rab1A on different lysates with Rabbit anti-Rab1A antibody (HA722021) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: A549 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C2C12 cell lysate
Lane 7: C6 cell lysate
Lane 8: Mouse brain tissue lysate
Lane 9: Mouse liver tissue lysate (low expression)
Lane 10: Rat brain tissue lysate
Lane 11: Rat liver tissue lysate (low expression)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: Lane 1-11 (left): 10 seconds; Lane 1-11 (right): 28 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722021) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Western blot analysis of Rab1A on different lysates with Rabbit anti-Rab1A antibody (HA722021) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution.
Lane 1: U-87 MG cell lysate
Lane 2: U-2 OS cell lysate
Lane 3: A549 cell lysate
Lane 4: HCT 116 cell lysate
Lane 5: NIH/3T3 cell lysate
Lane 6: C2C12 cell lysate
Lane 7: C6 cell lysate
Lane 8: Mouse brain tissue lysate
Lane 9: Mouse liver tissue lysate (low expression)
Lane 10: Rat brain tissue lysate
Lane 11: Rat liver tissue lysate (low expression)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: Lane 1-11 (left): 10 seconds; Lane 1-11 (right): 28 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722021) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.