Quinolones Recombinant Rabbit Monoclonal Antibody - BSA and Azide free [PSH0-35] (HA721339)
Catalog# HA721339
Rabbit Monoclonal to Quinolones
Tested for
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ELISA
Reacts with
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Species independent
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Competitive ELISA analysis of quinolones was performed by coating wells of a 96-well plate with 50 µl per well of quinolones-BSA diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with 1% BSA blocking buffer, and incubated with 100 µl per well of quinolones monoclonal antibody (HA721339) at concentration of 0. 5 µg/mL with serial diluted quinolones starting from a concentration of 20ug/ml for 1 hours at room temperature. The plate was washed and incubated with 50 µl per well of an HRP-conjugated Goat anti-Rabbit IgG secondary antibody (HA1001) at a dilution of 1:15,000 for one hour at room temperature. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.