Pyruvate Dehydrogenase E2 Recombinant Rabbit Monoclonal Antibody [JE34-38] (HA721267)
Catalog# HA721267
Rabbit Monoclonal to Pyruvate Dehydrogenase E2
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HL-60 cell lysate (20 µg/Lane)
Lane 2: Mouse heart tissue lysate (40 µg/Lane)
Predicted band size: 69 kDa
Observed band size: 60 kDa
Exposure time: 4 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721267) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Pyruvate Dehydrogenase E2 Recombinant Rabbit Monoclonal Antibody [JE34-38] (HA721267)
Immunogen
Synthetic peptide within human pyruvate dehydrogenase e2 aa 574-624/647.
Host
Rabbit
Positive Control
HL-60 cell lysate, Mouse heart tissue lysate, Mouse liver tissue lysate, Rat liver tissue lysate, HAP1 cell lysate, human testis tissue, mouse cerebellum tissue, rat brain tissue, rat hippocampus tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE34-38
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IHC-P
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1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 69 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Pyruvate Dehydrogenase E2
SYNONYMS
70 kDa mitochondrial autoantigen of primary biliary cirrhosis antibody;Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex antibody;Dihydrolipoamide S Acetyltransferase antibody;Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex antibody;dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial antibody;DLAT antibody;DLTA antibody;E2 antibody;E2 component of pyruvate dehydrogenase complex antibody;M2 antigen complex 70 kDa subunit antibody;mitochondrial antibody;ODP2_HUMAN antibody;PBC antibody;PDC E2 antibody;PDC-E2 antibody;PDCE2 antibody;Pyruvate dehydrogenase complex component E2 antibody;Pyruvate dehydrogenase complex E2 subunit antibody
SUBCELLULAR LOCATION
Mitochondrion matrix.
FUNCTION
Dihydrolipoyl transacetylase (or dihydrolipoamide acetyltransferase) is an enzyme component of the multienzyme pyruvate dehydrogenase complex. The pyruvate dehydrogenase complex is responsible for the pyruvate decarboxylation step that links glycolysis to the citric acid cycle. This involves the transformation of pyruvate from glycolysis into acetyl-CoA which is then used in the citric acid cycle to carry out cellular respiration. There are three different enzyme components in the pyruvate dehydrogenase complex. Pyruvate dehydrogenase (EC 1.2.4.1) is responsible for the oxidation of pyruvate, dihydrolipoyl transacetylase (this enzyme; EC 2.3.1.12) transfers the acetyl group to coenzyme A (CoA), and dihydrolipoyl dehydrogenase (EC 1.8.1.4) regenerates the lipoamide. Because dihydrolipoyl transacetylase is the second of the three enzyme components participating in the reaction mechanism for conversion of pyruvate into acetyl CoA, it is sometimes referred to as E2. In humans, dihydrolipoyl transacetylase enzymatic activity resides in the pyruvate dehydrogenase complex component E2 (PDCE2) that is encoded by the DLAT (dihydrolipoamide S-acetyltransferase) gene.
CITATIONS
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Hu Jiahe,et al
Engineered-Doping Strategy for Self-Sufficient Reactive Oxygen Species Blossom to Amplify Ferroptosis/Cuproptosis Sensibilization in Hepatocellular Carcinoma Treatment
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WB
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Human
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Citation
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Caili Chen,et al
Bovine serum albumin-bound homologous targeted nanoparticles for breast cancer combinatorial therapy
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WB
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Mouse
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Citation
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