PTP1B Mouse Monoclonal Antibody [D0-C7] (M1511-7)
Catalog# M1511-7
Mouse Monoclonal to PTP1B
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WB
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IF-Cell
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-PTP1B KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 50 kDa
Observed band size: 50 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (M1511-7) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Mouse monoclonal primary
Product Name
PTP1B Mouse Monoclonal Antibody [D0-C7] (M1511-7)
Immunogen
Recombinant protein within human ptp1b aa 1-250.
Host
Mouse
Positive Control
A431 cell lysate, Hela cell lysate, A549 cell lysate, HepG2 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, A431, human lung cancer tissue, mouse lung tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
D0-C7
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:2,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:200-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 50 kDa
Isotype
IgG1
TARGET
UNIPROT #
PROTEIN NAME
PTP1B
SYNONYMS
PTP1B antibody;Non receptor tyrosine phosphatase 1 antibody;Protein phosphotyrosylphosphatase 1B antibody;Protein tyrosine phosphatase 1B antibody;Protein tyrosine phosphatase non receptor type 1 antibody;Protein tyrosine phosphatase placental antibody;Protein-tyrosine phosphatase 1B antibody;PTN1_HUMAN antibody;PTP 1B antibody;PTP-1B antibody;PTPN 1 antibody;PTPN1 antibody;Tyrosine protein phosphatase non receptor type 1 antibody;Tyrosine-protein phosphatase non-receptor type 1 antibody
SEQUENCE SIMILARITIES
Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily.
TISSUE SPECIFICITY
Expressed in keratinocytes (at protein level).
POST-TRANSLATIONAL MODIFICATION
Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a sulfenamide cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.; Ser-50 is the major site of phosphorylation as compared to Ser-242 and Ser-243. Activated by phosphorylation at Ser-50.; S-nitrosylation of Cys-215 inactivates the enzyme activity.; Sulfhydration at Cys-215 following endoplasmic reticulum stress inactivates the enzyme activity, promoting EIF2AK3/PERK activity.
SUBCELLULAR LOCATION
Endoplasmic reticulum membrane
FUNCTION
The phosphorylation of proteins at tyrosine residues has long been recognized as an important regulatory component of signal transduction. This is a reversible process, involving both enzymes that phosphorylate proteins on tyrosine residues as well as a rapidly expanding family of protein tyrosine phosphatases. These latter enzymes bear little resemblance to either the protein serine and protein threonine phosphatases or to the acid and alkaline phosphatases. In most tissues, the major PTPase is a vanadate- and molybdate-sensitive protein. On the basis of sequence analysis, PTP1B (PTPase 1B) expressed in human placenta exhibits similarities both with the common leukocyte antigen (CD45) and with LAR, a homolog of the neural adhesion molecule (NCAM). PTP1B is synthesized as a 435 amino acid precursor protein which is cleaved to generate the active 321 amino acid enzyme.
CITATIONS
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Jingmin Zhou,Junbo Ge
Angiotensin II induces apoptosis of cardiac microvascular endothelial cells via regulating PTP1B/PI3K/Akt pathway.
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WB
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CMECs
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Citation
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