PTBP1 Recombinant Rabbit Monoclonal Antibody [JU41-20] (ET7106-58)
Catalog# ET7106-58
Rabbit Monoclonal to PTBP1
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: K562 cell lysate
Lane 2: Jurkat cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7106-58) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PTBP1 Recombinant Rabbit Monoclonal Antibody [JU41-20] (ET7106-58)
Immunogen
Synthetic peptide within human ptbp1 aa 20-69 / 531.
Host
Rabbit
Positive Control
K562 cell lysate, Jurkat cell lysate, human tonsil tissue, human skin tissue, human kidney tissue, mouse colon tissue, human lymph nodes tissue,mouse large intestine tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU41-20
RRID
APPLICATION DILUTION
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WB
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1:500-2,000
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
57 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PTBP1
SYNONYMS
57 kDa RNA binding protein PPTB 1 antibody;57 kDa RNA-binding protein PPTB-1 antibody;Heterogeneous nuclear ribonucleoprotein I antibody;Heterogeneous Nuclear Ribonucleoprotein Polypeptide I antibody;hnRNP I antibody;HNRNP-I antibody;HNRNPI antibody;HNRPI antibody;MGC10830 antibody;MGC8461 antibody;Polypyrimidine tract binding protein (heterogeneous nuclear ribonucleoprotein I) antibody;Polypyrimidine Tract Binding Protein 1 antibody;Polypyrimidine tract binding protein antibody;Polypyrimidine tract-binding protein 1 antibody;pPTB antibody;PTB 1 antibody;PTB 2 antibody;PTB 3 antibody;PTB 4 antibody;PTB antibody;PTB T antibody;PTB1 antibody;PTB2 antibody;PTB3 antibody;PTB4 antibody;PTBP 1 antibody;PTBP1 antibody;PTBP1_HUMAN antibody;PTBT antibody;RNA Binding Protein antibody
SUBCELLULAR LOCATION
Nucleus.
FUNCTION
Plays a role in pre-mRNA splicing and in the regulation of alternative splicing events. Activates exon skipping of its own pre-mRNA during muscle cell differentiation. Binds to the polypyrimidine tract of introns. May promote RNA looping when bound to two separate polypyrimidine tracts in the same pre-mRNA. May promote the binding of U2 snRNP to pre-mRNA. Cooperates with RAVER1 to modulate switching between mutually exclusive exons during maturation of the TPM1 pre-mRNA. Represses the splicing of MAPT/Tau exon 10. In case of infection by picornaviruses, binds to the viral internal ribosome entry site (IRES) and stimulates the IRES-mediated translation.
CITATIONS
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Rao Zichen
HNRNP I promotes IRAK1 degradation to reduce podocyte apoptosis and inflammatory response alleviating renal injury in diabetic nephropathy
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WB
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Human
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Citation
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Hailei Shan,et al
Bone marrow mesenchymal stem cells with PTBP1 knockdown protect against cerebral ischemia-reperfusion injury by inhibiting ferroptosis via the JNK/P38 pathway in rats
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WB
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Citation
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