PSMA5 Rabbit Polyclonal Antibody (ER2001-68)
Catalog# ER2001-68
Rabbit Polyclonal to PSMA5
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WB
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IHC-P
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Human
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Mouse
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Rat
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: HEK-293 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: Mouse cerebellum tissue lysate (40 µg/Lane)
Lane 5: Mouse liver tissue lysate (40 µg/Lane)
Predicted band size: 26 kDa
Observed band size: 26 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER2001-68) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
PSMA5 Rabbit Polyclonal Antibody (ER2001-68)
Immunogen
Recombinant protein within human psma5 aa 1-200.
Host
Rabbit
Positive Control
HeLa cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, mouse cerebellum tissue lysate, mouse liver tissue lysate, human liver carcinoma tissue, human colon carcinoma tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000-1:5,000
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IHC-P
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1:100-1:500
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 26 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PSMA5
SYNONYMS
Macropain zeta chain antibody;Multicatalytic endopeptidase complex zeta chain antibody;Proteasome (prosome macropain) subunit alpha type 5 antibody;Proteasome alpha5 subunit antibody;Proteasome component 5 antibody;Proteasome subunit alpha type 5 antibody;Proteasome subunit alpha type-5 antibody;Proteasome subunit zeta antibody;Proteasome zeta chain antibody;PSA5_HUMAN antibody;PSC5 antibody;PSMA5 antibody;ZETA antibody
SUBCELLULAR LOCATION
Cytoplasm, Nucleus, Proteasome.
FUNCTION
Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).
CITATIONS
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Duan, J., Li, W., Shu, X., Yang, B., He, X., & Guo, X.
Conserved Mitotic Phosphorylation of a Proteasome Subunit Regulates Cell Proliferation
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WB
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Human
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Citation
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