PRODUCT CODE: ET1705-90

PSD93 Recombinant Rabbit Monoclonal Antibody [JM41-04] (ET1705-90)

  • Recombinant

Applications

  • WB

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PSD93 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: SH-SY5Y cell lysate
  • Western blot analysis of PSD93 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: Hela cell lysate<br />
Lane 2: SH-SY5Y cell lysate
  • ICC staining of PSD93 in SH-SY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ET1705-90, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
Western blot analysis of PSD93 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ET1705-90, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Hela cell lysate
Lane 2: SH-SY5Y cell lysate

Applications

  • WB

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

PSD93 Recombinant Rabbit Monoclonal Antibody [JM41-04] (ET1705-90)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Hela cell lysate, SH-SY5Y cell lysate, SH-SY5Y.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM41-04

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A affinity purified.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • FC

  • 1:50-1:100

  • IP

  • 1:10-1:50

TARGET

UNIPROT #

PROTEIN NAME

PSD93

SYNONYMS

Channel associated protein of synapse 110 antibody; Channel associated protein of synapses 110kD antibody; Channel-associated protein of synapse-110 antibody; Chapsyn 110 antibody; Chapsyn-110 antibody; Chapsyn110 antibody; Discs, large homolog 2 (Drosophila) antibody; Disks large homolog 2 antibody; DKFZp781D1854 antibody; DKFZp781E0954 antibody; Dlg 2 antibody; dlg2 antibody; DLG2_HUMAN antibody; Dlgh 2 antibody; Dlgh2 antibody; FLJ37266 antibody; Gm1197 antibody; MGC131811 antibody; Postsynaptic density protein PSD 93 antibody; Postsynaptic density protein PSD-93 antibody; Postsynaptic density protein PSD93 antibody; PSD 93 antibody; PSD93 antibody

SEQUENCE SIMILARITIES

Belongs to the MAGUK family.

POST-TRANSLATIONAL MODIFICATION

Palmitoylation of isoform 1 is not required for targeting to postsynaptic density.

SUBCELLULAR LOCATION

Cell membrane, postsynaptic density, synapse, Membrane, axon, perikaryon.

FUNCTION

Required for perception of chronic pain through NMDA receptor signaling. Regulates surface expression of NMDA receptors in dorsal horn neurons of the spinal cord. Interacts with the cytoplasmic tail of NMDA receptor subunits as well as inward rectifying potassium channels. Involved in regulation of synaptic stability at cholinergic synapses. Part of the postsynaptic protein scaffold of excitatory synapses.