PRODUCT CODE: 0905-4

Protocadherin Fat 1 Rabbit Polyclonal Antibody (0905-4)

Applications

  • WB

REACTIVITY

  • Human

Western blot analysis on Fat1 recombinant protein at 1:5,000.
  • Western blot analysis on Fat1 recombinant protein at 1:5,000.
  • Immunohistochemical analysis of paraffin-embedded human Colon cancer tissue using anti-FAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0905-4, 1/100) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Immunohistochemical analysis of paraffin-embedded human Stomach tissue using anti-FAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (0905-4, 1/100) for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Western blot analysis on Fat1 recombinant protein at 1:5,000.

Applications

  • WB

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Protocadherin Fat 1 Rabbit Polyclonal Antibody (0905-4)

Immunogen

Synthetic peptide within human protocadherin fat 1 aa 238-287 / 4,588.

Host

Rabbit

Positive Control

Recombinant protein

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Immunogen affinity purified

MOLECULAR WEIGHT

50kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:2,000-5,000

  • ICC

  • 1:100

TARGET

UNIPROT #

PROTEIN NAME

Protocadherin Fat 1

SYNONYMS

Cadherin family member 7 antibody; Cadherin family member 7 precursor antibody; Cadherin ME5 antibody; Cadherin related tumor suppressor antibody; Cadherin related tumor suppressor homolog antibody; Cadherin related tumor suppressor homolog precursor antibody; Cadherin related tumor suppressor homolog precursor (FAT protein homolog) antibody; Cadherin-related tumor suppressor homolog antibody; CDHF 7 antibody; CDHF7 antibody; FAT 1 antibody; FAT tumor suppressor 1 antibody; FAT tumor suppressor homolog 1 antibody; FAT1 antibody; FAT1_HUMAN antibody; hFat 1 antibody; hFAT antibody; hFat1 antibody; Homolog of Drosophila FAT protein antibody; Homolog of Drosophila FAT protein precursor antibody; Homolog of Drosophila tumor suppressor FAT precursor antibody; ME 5 antibody; ME5 antibody; nuclear form antibody; Protein fat homolog antibody

TISSUE SPECIFICITY

Expressed in many epithelial and some endothelial and smooth muscle cells.

POST-TRANSLATIONAL MODIFICATION

Undergoes proteolytic cleavage. The extracellular domain is cleaved off and the cytoplasmic domain (about 400 AA) shuttles to the nucleus.

SUBCELLULAR LOCATION

Cell membrane

FUNCTION

Mammalian FAT1 is a large transmembrane protein of the protocadherin superfamily ?rst identi?ed in Drosophila melanogaster. FAT1 is widely expressed in mammalian tissues, and it is particularly enriched in kidney glomerular epithelial cells. Human Protocadherin Fat1 is a single-pass type I membrane protein, contains 4950 amino acids. It may play an important role in regulating growth and cell adhesion.