FAT1 Rabbit Polyclonal Antibody (0905-4)

UNIPROT #

Q14517

PROTEIN NAME

FAT1

SYNONYMS

Cadherin family member 7 antibody;Cadherin family member 7 precursor antibody;Cadherin ME5 antibody;Cadherin related tumor suppressor antibody;Cadherin related tumor suppressor homolog antibody;Cadherin related tumor suppressor homolog precursor antibody;Cadherin related tumor suppressor homolog precursor (FAT protein homolog) antibody;Cadherin-related tumor suppressor homolog antibody;CDHF 7 antibody;CDHF7 antibody;FAT 1 antibody;FAT tumor suppressor 1 antibody;FAT tumor suppressor homolog 1 antibody;FAT1 antibody;FAT1_HUMAN antibody;hFat 1 antibody;hFAT antibody;hFat1 antibody;Homolog of Drosophila FAT protein antibody;Homolog of Drosophila FAT protein precursor antibody;Homolog of Drosophila tumor suppressor FAT precursor antibody;ME 5 antibody;ME5 antibody;nuclear form antibody;Protein fat homolog antibody

TISSUE SPECIFICITY

Expressed in many epithelial and some endothelial and smooth muscle cells.

POST-TRANSLATIONAL MODIFICATION

Undergoes proteolytic cleavage. The extracellular domain is cleaved off and the cytoplasmic domain (about 400 AA) shuttles to the nucleus.

SUBCELLULAR LOCATION

Cell membrane, Nucleus.

FUNCTION

Protocadherin FAT1 is a protein that in humans is encoded by the FAT1 gene. This gene is an ortholog of the Drosophila fat gene, which encodes a tumor suppressor essential for controlling cell proliferation during Drosophila development. The gene product is a member of the cadherin superfamily, a group of integral membrane proteins characterized by the presence of cadherin-type repeats. This gene is expressed at high levels in a number of fetal epithelia. Transcript variants derived from alternative splicing and/or alternative promoter usage exist, but they have not been fully described. It has been shown that the EVH motifs in the cytoplasmic tail of mouse Fat1 interact with Ena/VASP and ablation of Fat1 by RNAi leads to decreased cell migration of rat epithelial cells. The cytoplasmic tail of Fat1 has also been shown to bind the transcriptional repressor Atrophin in rat vascular smooth muscle cells. At the carboxyl terminus of FAT1 lies a PDZ domain (PSD95/Dlg1/ZO-1) ligand motif (-HTEV). Zebrafish Fat1 was found to bind the protein scribble and regulate Hippo signalling. Using the human SHSY5Y cell line as a model of neuronal differentiation, human FAT1 was shown to regulate Hippo kinase components with loss of FAT1 leading to nucleocytoplasmic relocation of TAZ and enhanced transcription of the Hippo target gene CTGF. The same study also showed FAT1 was able to regulate TGF-beta signaling. FAT1 has been found to bind beta-catenin and regulate Wnt-signaling in colorectal cancer. Human FAT1 was found to bind glypican-3 (GPC3) and regulate cell migration in liver cancer cells.