PRODUCT CODE: ET7107-59

Proteasome 20S C2 Recombinant Rabbit Monoclonal Antibody [JB89-30] (ET7107-59)

  • Recombinant

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

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Western blot analysis of Proteasome 20S C2 on different lysates using anti-Proteasome 20S C2 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3M<br />
    Lane 2: K562 <br />
  Lane 3: Rat testis<br />
 Lane 4: Mouse testis <br />
   Lane 5: Mouse colon
  • Western blot analysis of Proteasome 20S C2 on different lysates using anti-Proteasome 20S C2 antibody at 1/500 dilution.<br />
 Positive control:<br />
 Lane 1: PC-3M<br />
    Lane 2: K562 <br />
  Lane 3: Rat testis<br />
 Lane 4: Mouse testis <br />
   Lane 5: Mouse colon
  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.
  • Flow cytometric analysis of K562 cells with Proteasome 20S C2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Western blot analysis of Proteasome 20S C2 on different lysates using anti-Proteasome 20S C2 antibody at 1/500 dilution.
Positive control:
Lane 1: PC-3M
Lane 2: K562
Lane 3: Rat testis
Lane 4: Mouse testis
Lane 5: Mouse colon

Applications

  • WB

  • IHC-P

  • FC

  • IP

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Proteasome 20S C2 Recombinant Rabbit Monoclonal Antibody [JB89-30] (ET7107-59)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Rat testis tissue, human liver cancer tissue, human pancreas tissue, mouse cerebellum tissue, K562, PC-3M, mouse testis tissue lysate, mouse colon tissue lysate.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JB89-30

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

30 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • IHC-P

  • 1:50-1:200

  • FC

  • 1:50-1:100

  • ICC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

Proteasome 20S C2

SYNONYMS

30 kDa prosomal protein antibody; HC 2 antibody; HC2 antibody; Macropain subunit C2 antibody; Macropain subunit nu antibody; MGC14542 antibody; MGC14575 antibody; MGC14751 antibody; MGC1667 antibody; MGC21459 antibody; MGC22853 antibody; MGC23915 antibody; Multicatalytic endopeptidase complex subunit C2 antibody; NU antibody; PROS 30 antibody; PROS-30 antibody; PROS30 antibody; Proteasome (prosome macropain) subunit alpha type 1 antibody; Proteasome alpha 1 subunit antibody; Proteasome component C2 antibody; Proteasome nu chain antibody; Proteasome subunit alpha type 1 antibody; Proteasome subunit alpha type I antibody; Proteasome subunit alpha type-1 antibody; Proteasome subunit nu antibody; Protein P30 33K antibody; PSA1_HUMAN antibody; PSC 2 antibody; PSC2 antibody; PSMA 1 antibody; psmA1 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase T1A family.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

Component of the 20S core proteasome complex involved in the proteolytic degradation of most intracellular proteins. This complex plays numerous essential roles within the cell by associating with different regulatory particles. Associated with two 19S regulatory particles, forms the 26S proteasome and thus participates in the ATP-dependent degradation of ubiquitinated proteins. The 26S proteasome plays a key role in the maintenance of protein homeostasis by removing misfolded or damaged proteins that could impair cellular functions, and by removing proteins whose functions are no longer required. Associated with the PA200 or PA28, the 20S proteasome mediates ubiquitin-independent protein degradation. This type of proteolysis is required in several pathways including spermatogenesis (20S-PA200 complex) or generation of a subset of MHC class I-presented antigenic peptides (20S-PA28 complex).