pro Caspase-9 Recombinant Rabbit Monoclonal Antibody [JJ08-05] (ET1701-22)

UNIPROT #

P55211

PROTEIN NAME

pro Caspase-9

SYNONYMS

Caspase9;APAF-3 antibody;APAF3 antibody;Apoptotic protease Mch-6 antibody;Apoptotic protease-activating factor 3 antibody;CASP-9 antibody;CASP9 antibody;CASP9_HUMAN antibody;Caspase-9 subunit p10 antibody;Caspase-9 antibody;Caspase 9 antibody;Caspase9 antibody;ICE-LAP6 antibody;ICE-like apoptotic protease 6 antibody;ICELAP6 antibody;MCH6 antibody

SEQUENCE SIMILARITIES

Belongs to the peptidase C14A family.

TISSUE SPECIFICITY

Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes.

DEVELOPMENTAL STAGE

Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart.

POST-TRANSLATIONAL MODIFICATION

Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events.; Phosphorylated at Thr-125 by MAPK1/ERK2. Phosphorylation at Thr-125 is sufficient to block caspase-9 processing and subsequent caspase-3 activation. Phosphorylation on Tyr-153 by ABL1/c-Abl; occurs in the response of cells to DNA damage.

SUBCELLULAR LOCATION

Cytosol,Nucleus.

FUNCTION

Caspase-9 is an enzyme that in humans is encoded by the CASP9 gene. It is an initiator caspase, critical to the apoptotic pathway found in many tissues. Caspase-9 belongs to a family of caspases, cysteine-aspartic proteases involved in apoptosis and cytokine signalling. Apoptotic signals cause the release of cytochrome c from mitochondria and activation of apaf-1 (apoptosome), which then cleaves the pro-enzyme of caspase-9 into the active dimer form. Correct caspase-9 function is required for apoptosis, leading to the normal development of the central nervous system. Caspase-9 has multiple additional cellular functions that are independent of its role in apoptosis. Nonapoptotic roles of caspase-9 include regulation of necroptosis, cellular differentiation, innate immune response, sensory neuron maturation, mitochondrial homeostasis, corticospinal circuit organization, and ischemic vascular injury. Without correct function, abnormal tissue development can occur leading to abnormal function, diseases and premature death. Caspase-9 loss-of-function mutations have been associated with immunodeficiency/lymphoproliferation, neural tube defects, and Li-Fraumeni-like syndrome. Increased caspase-9 activity is implicated in the progression of amyotrophic lateral sclerosis, retinal detachment, and slow-channel syndrome, as well as various other neurological, autoimmune, and cardiovascular disorders.