PRODUCT CODE: EM31103

PRMT5 Mouse Monoclonal Antibody [7-C2] (EM31103)

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PRMT5 on mouse kidney lysates using anti-PRMT5 antibody at 1/500 dilution.
  • Western blot analysis of PRMT5 on mouse kidney lysates using anti-PRMT5 antibody at 1/500 dilution.
  • Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRMT5 antibody. Counter stained with hematoxylin.
Western blot analysis of PRMT5 on mouse kidney lysates using anti-PRMT5 antibody at 1/500 dilution.

Applications

  • WB

  • IHC-P

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PRMT5 Mouse Monoclonal Antibody [7-C2] (EM31103)

Immunogen

Synthetic peptide within human prmt5 aa 1-50 / 637.

Host

Mouse

Positive Control

Human kidney tissue, human liver tissue, mouse kidney tissue, mouse liver tissue, rat kidney tissue, rat liver tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7-C2

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

72 kDa

Isotype

IgG2a

APPLICATION DILUTION

  • WB

  • 1:1,000

  • IHC-P

  • 1:100-1:200

TARGET

UNIPROT #

PROTEIN NAME

PRMT5

SYNONYMS

72 kDa ICln binding protein antibody; 72 kDa ICln-binding protein antibody; ANM5_HUMAN antibody; Histone synthetic lethal 7, S. cerevisiae, homolog of antibody; Histone-arginine N-methyltransferase PRMT5 antibody; HMT1 hnRNP methyltransferase like 5 antibody; HOMOLOG OF; SKB1 antibody; HRMT1L5 antibody; IBP72 antibody; Jak-binding protein 1 antibody; JBP 1 antibody; JBP1 antibody; PRMT 5 antibody; PRMT5 antibody; Protein arginine methyltransferase 5 antibody; Protein arginine N methyltransferase 5 antibody; Protein arginine N methyltransferase 5 N terminally processed antibody; Protein arginine N-methyltransferase 5 antibody; S. POMBE antibody; S. POMBE HOMOLOG OF; SKB1 antibody; SHK1 KINASE BINDING PROTEIN 1 antibody; Shk1 kinase binding protein 1 homolog antibody; Shk1 kinase-binding protein 1 homolog antibody; Shk1 kinase/binding protein 1, S. pombe, homolog of antibody; SKB 1 antibody; SKB1 antibody; SKB1 homolog antibody; SKB1: SKB1 homolog (S. pombe) antibody; SKB1Hs antibody

SEQUENCE SIMILARITIES

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.

TISSUE SPECIFICITY

Ubiquitous.

SUBCELLULAR LOCATION

Nucleus, cytoplasm.

FUNCTION

The formation of the spliceosome includes the assembly of Sm proteins inan ordered manner onto snRNAs. This process is mediated by the survivalof a motor neuron (SMN) protein, and is enhanced by modification of specificArginine residues in the Sm proteins to symmetrical dimethylarginines(sDMAs). sDMA modification of Sm proteins is catalyzed by the methylosome,a complex comprised of the type II methyltransferase PRMT5 (alsodesignated JAK-binding protein 1, JBP1), pICln and two novel factors. PRMT5binds the Sm proteins via their Arginine- and Glycine-rich (RG) domains, whilepICln binds the Sm domains. PRMT5 is a distinct member of the protein-Arginine methyltransferase (PRMT) family, and predominantly localizes tothe cytoplasm in a wide variety of tissues. PRMT5 also associates specificallywith the transcription start site region of the cyclin E1 promoter and, therefore,is involved in the control of transcription and proliferation.The geneencoding human PRMT5 maps to chromosome 14q11.2.