PRDX6 Mouse Monoclonal Antibody [7G1] (EM1701-73)

Western blot analysis of PRDX6 on PC-3M (1) and K562 (2) using anti-PRDX6 antibody at 1/500 dilution.

Applications

WB
IHC-P
ICC
FC

REACTIVITY

Human

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PRDX6 Mouse Monoclonal Antibody [7G1] (EM1701-73)

Immunogen

Recombinant protein.

Host

Mouse

Positive Control

PC-3M, K562, A431, SiHa, human liver tissue, human colon cancer tissue, human kidney tissue, human placenta tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

7G1

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

25 kDa

Isotype

IgG1

APPLICATION DILUTION

WB
1:500

ICC
1:50-1:200

IHC-P
1:100-1:400

FC
1:50-1:100

TARGET

UNIPROT #

P30041

PROTEIN NAME

PRDX6

SYNONYMS

1 Cys antibody; 1 Cys peroxiredoxin antibody; 1 Cys PRX antibody; 1 cysPrx antibody; 1-Cys peroxiredoxin antibody; 1-Cys PRX antibody; 24 kDa protein antibody; 9430088D19Rik antibody; AA690119 antibody; Acidic calcium independent phospholipase A2 antibody; Acidic calcium-independent phospholipase A2 antibody; aiPLA2 antibody; Antioxidant protein 2 antibody; AOP2 antibody; Aop2 rs3 antibody; Brp 12 antibody; Ciliary body glutathione peroxidase antibody; CP 3 antibody; EC 1.11.1.15 antibody; EC 1.11.1.7 antibody; EC 3.1.1. antibody; Epididymis secretory sperm binding protein Li 128m antibody; GPx antibody; HEL S 128m antibody; KIAA0106 antibody; Liver 2D page spot 40 antibody; Ltw4 antibody; Lvtw 4 antibody; MGC46173 antibody; mKIAA0106 antibody; Non selenium glutathione peroxidase antibody; Non-selenium glutathione peroxidase antibody; NSGPx antibody; ORF06 antibody; OTTHUMP00000032693 antibody; p29 antibody; Peroxiredoxin-6 antibody; Peroxiredoxin6 antibody; PHGPx antibody; Phospholipase A2 lysosomal antibody; PLA2 antibody; PRDX 6 antibody; Prdx5 antibody; PRDX6 antibody; Prdx6 rs3 antibody; PRDX6_HUMAN antibody; PRX antibody; Red blood cells page spot 12 antibody; Thiol specific antioxidant protein antibody

SEQUENCE SIMILARITIES

Belongs to the peroxiredoxin family. Prx6 subfamily.

POST-TRANSLATIONAL MODIFICATION

Irreversibly inactivated by overoxidation of Cys-47 to sulfinic acid (Cys-SO(2)H) and sulfonic acid (Cys-SO(3)H) forms upon oxidative stress.; Phosphorylation at Thr-177 by MAP kinases increases the phospholipase activity of the enzyme.

SUBCELLULAR LOCATION

Cytoplasm. Lysosome.

FUNCTION

The peroxiredoxin (PRX) family comprises six antioxidant proteins, PRX I, II, III, IV, V and VI, which protect cells from reactive oxygen species (ROS) by preventing the metal-catalyzed oxidation of enzymes. The PRX proteins primarily utilize thioredoxin as the electron donor for antioxidation, although they are fairly promiscuous with regard to the hydroperoxide substrate. In addition to protection from ROS, peroxiredoxins are also involved in cell proliferation, differentiation and gene expression. PRX I, II, IV and VI show diffuse cytoplasmic localization, while PRX III and V exhibit distinct mitochondrial localization.PRX VI, a 1-Cys peroxiredoxin (also known as antioxidant protein 2 or AOP2), is highly expressed in most tissues, particularly in epithelial cells. Localized to the cell cytosol, PRX VI functions independently of other peroxiredoxins and antioxidant proteins, specializing in antioxidant defense, lung phospholipid metabolism and protection of keratinocytes from cell death induced by reactive oxygen species.

Goat IgG Light chain Mouse Monoclonal Antibody [B8-C4-G4] (M0910-2)

Applications

WB
ELISA
IP

REACTIVITY

Goat

Western blot analysis of Goat IgG Light chain on Goat IgG protein lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (M0910-2, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Thymidine phosphorylase Mouse Monoclonal Antibody [A1A8] (HA600036)

Applications

REACTIVITY

Human

Western blot analysis of Thymidine phosphorylase on human lung tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600036, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

MMAF Mouse Monoclonal Antibody [A5G11] (HA600035)

Applications

ELISA

REACTIVITY

The binding activity of HA600035 with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody (HA600035) for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600035) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

MMAF Mouse Monoclonal Antibody [A5G12] (HA600034)

Applications

ELISA

REACTIVITY

The binding activity of (HA600034) with MMAF-ADC, MMAF-BSA, MMAE-BSA, DM1-ADC and BSA protein at 1 μg/ml overnight at 4℃. Then blocked with 1% BSA for 1 hour at 37℃, and incubated with the primary antibody () for 1 hour at 25℃.<br />
<br />
Mouse anti-MMAF Ab (HA600034) can bind to MMAF-ADC or MMAF-BSA but not BSA or MMAE-BSA.

IGF1R Mouse Monoclonal Antibody [D5-G2] (HA600033)

Applications

REACTIVITY

Human

Western blot analysis of IGF1R on 293 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (HA600033, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.

Pan-Actin Mouse Monoclonal Antibody [A2E1] (HA600032)

Applications

WB
IHC-P

REACTIVITY

Human
Mouse
Rat