PRAK / MK5 Recombinant Rabbit Monoclonal Antibody [PSH14-06] (HA723596)
Catalog# HA723596
Rabbit Monoclonal to PRAK / MK5
Tested for
-
WB
-
IP
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of PRAK / MK5 on different lysates with Rabbit anti-PRAK / MK5 antibody (HA723596) at 1/5,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: COS-1 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: MEF cell lysate
Lane 9: C6 cell lysate
Lane 10: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 52/66 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723596) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: MDA-MB-231 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: COS-1 cell lysate
Lane 7: NIH/3T3 cell lysate
Lane 8: MEF cell lysate
Lane 9: C6 cell lysate
Lane 10: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 54 kDa
Observed band size: 52/66 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA723596) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.