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PRODUCT CODE: ER1902-24

PPAR delta Rabbit Polyclonal Antibody (ER1902-24)

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of PPAR delta on mouse colon tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of PPAR delta on mouse colon tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Flow cytometric analysis of PPAR delta was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-24, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; blcak).
Western blot analysis of PPAR delta on mouse colon tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-24, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • FC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PPAR delta Rabbit Polyclonal Antibody (ER1902-24)

Immunogen

Recombinant protein within human ppard aa 260-441.

Host

Rabbit

Positive Control

Mouse colon tissue lysates, SHSY5Y.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein affinity purified.

MOLECULAR WEIGHT

Predicted band size 50 kDa.

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1000

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

Q03181

PROTEIN NAME

PPAR delta

SYNONYMS

FAAR antibody; MGC3931 antibody; NR1C2 antibody; NUC1 antibody; NUCI antibody; NUCII antibody; Nuclear hormone receptor 1 antibody; Nuclear receptor subfamily 1 group C member 2 antibody; Peroxisome proliferative activated receptor delta antibody; Peroxisome proliferator-activated receptor beta (PPAR-beta) antibody; Peroxisome proliferator-activated receptor beta antibody; Peroxisome proliferator-activated receptor delta antibody; PPAR beta antibody; PPAR-beta antibody; PPAR-delta antibody; PPAR-ß antibody; PPARB antibody; ppard antibody; PPARD_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the nuclear hormone receptor family. NR1 subfamily.

TISSUE SPECIFICITY

Ubiquitous with maximal levels in placenta and skeletal muscle.

SUBCELLULAR LOCATION

Nucleus.

FUNCTION

PPARδ is a nuclear hormone receptor that governs a variety of biological processes and may be involved in the development of several chronic diseases, including diabetes, obesity, atherosclerosis, and cancer.In muscle PPAR-β/δ expression is increased by exercise, resulting in increased oxidative (fat-burning) capacity and an increase in type I fibers. Both PPAR-β/δ and AMPK agonists are regarded as exercise mimetics. In adipose tissue PPAR-β/δ increases both oxidation as well as uncoupling of oxidative phosphorylation.PPARδ may function as an integrator of transcription repression and nuclear receptor signaling. It activates transcription of a variety of target genes by binding to specific DNA elements. Well described target genes of PPARδ include PDK4, ANGPTL4, PLIN2, and CD36. The expression of this gene is found to be elevated in colorectal cancer cells. The elevated expression can be repressed by adenomatosis polyposis coli (APC), a tumor suppressor protein involved in the APC/beta-catenin signaling pathway. Knockout studies in mice suggested the role of this protein in myelination of the corpus callosum, epidermal cell proliferation, and glucose and lipid metabolism.This protein has been shown to be involved in differentiation, lipid accumulation, directional sensing, polarization, and migration in keratinocytes.