PP2A alpha + beta Recombinant Rabbit Monoclonal Antibody [SN70-08] (ET1611-54)
Catalog# ET1611-54
Rabbit Monoclonal to PP2A alpha + beta
-
WB
-
IF-Cell
-
IHC-P
-
Human
-
Mouse
-
Rat
-
Zebrafish
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: A431 cell lysate (20 µg/Lane)
Lane 4: NIH/3T3 cell lysate (20 µg/Lane)
Lane 5: PC-12 cell lysate (20 µg/Lane)
Lane 6: Mouse brain tissue lysate (20 µg/Lane)
Lane 7: Rat brain tissue lysate (20 µg/Lane)
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 6 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1611-54) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PP2A alpha + beta Recombinant Rabbit Monoclonal Antibody [SN70-08] (ET1611-54)
Immunogen
Synthetic peptide within human pp2a alpha aa 260-309 / 309.
Host
Rabbit
Positive Control
HeLa cell lysate, A549 cell lysate, A431 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate, 293T cell lysate, F9 cell lysate, hybrid fish (crucian-carp) brain tissue lysates, A431, NIH/3T3, PC-12, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SN70-08
RRID
APPLICATION DILUTION
-
WB
-
1:2,000
-
IF-Cell
-
1:50-1:200
-
IHC-P
-
1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 36 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PP2A alpha + beta
SYNONYMS
PP2A A antibody;PP2A alpha antibody;PP2A B antibody;PP2A beta antibody;PP2A-alpha antibody;PP2AA_HUMAN antibody;PP2Ac antibody;PP2CB antibody;PPP2CA antibody;PPP2CB antibody;Protein phosphatase 2 catalytic subunit alpha isoform antibody;Protein phosphatase 2 catalytic subunit beta isoform antibody;Replication protein C antibody;RP C antibody;RP-C antibody;Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody;Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody;Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody
SEQUENCE SIMILARITIES
Belongs to the PPP phosphatase family. PP-1 subfamily.
POST-TRANSLATIONAL MODIFICATION
Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.; Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.; May be monoubiquitinated by NOSIP.
SUBCELLULAR LOCATION
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Nucleus.
FUNCTION
The catalytic subunit of protein phosphatase 2A (PP2A) is inactivated by in vitro phosphorylation of Tyr-307 by receptor and nonreceptor protein tyrosine kinases. The catalytic subunit of PP2A is phosphorylated by tyrosine-specific protein kinases and associates with a variety of regulatory subunits. Phosphorylation is enhanced in the presence of the phosphatase inhibitor okadaic acid, consistent with an autodephosphorylation reaction. Phosphorylation is catalyzed by p60v-src, p56lck, epidermal growth factor receptors, and insulin receptors. Transient deactivation of PP2A might enhance transmission of cellular signals through kinase cascades within cells. In eukaryotes, the phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the protein phosphatases.
CITATIONS
-
Dr. Lunzhi Dai,Dr. Xiaoting Wu
Proteomic Maps of Human Gastrointestinal Stromal Tumor Subgroups.
-
IHC
-
Human Gastrointestinal Stromal Tumor Subgroups
-
Citation
-
