PRODUCT CODE: M1603-4

PP2A(alpha+beta) Mouse Monoclonal Antibody [A0-G7] (M1603-4)

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of PP2A(alpha+beta) on A431 cell lysate using anti-PP2A(alpha+beta) antibody at 1/1000 dilution.
  • Western blot analysis of PP2A(alpha+beta) on A431 cell lysate using anti-PP2A(alpha+beta) antibody at 1/1000 dilution.
  • ICC staining PP2A(alpha+beta) (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PP2A(alpha+beta) (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PP2A(alpha+beta) (green) in SHG-44 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Western blot analysis of PP2A(alpha+beta) on A431 cell lysate using anti-PP2A(alpha+beta) antibody at 1/1000 dilution.

Applications

  • WB

  • ICC

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PP2A(alpha+beta) Mouse Monoclonal Antibody [A0-G7] (M1603-4)

Immunogen

Peptide

Host

Mouse

Positive Control

A431 cell lysate, Hela, MCF-7, SHG-44.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

A0-G7

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Peptide affinity purified

MOLECULAR WEIGHT

35 kDa

Isotype

IgG1

APPLICATION DILUTION

  • WB

  • 1:2,000-1:5,000

  • ICC

  • 1:100-1:500

  • FC

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

PP2A(alpha+beta)

SYNONYMS

PP2A A antibody; PP2A alpha antibody; PP2A B antibody; PP2A beta antibody; PP2A-alpha antibody; PP2AA_HUMAN antibody; PP2Ac antibody; PP2CB antibody; PPP2CA antibody; PPP2CB antibody; Protein phosphatase 2 catalytic subunit alpha isoform antibody; Protein phosphatase 2 catalytic subunit beta isoform antibody; Replication protein C antibody; RP C antibody; RP-C antibody; Serine/threonine protein phosphatase 2A catalytic subunit alpha isoform antibody; Serine/threonine protein phosphatase 2A catalytic subunit beta isoform antibody; Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform antibody

SEQUENCE SIMILARITIES

Belongs to the PPP phosphatase family. PP-1 subfamily.

POST-TRANSLATIONAL MODIFICATION

Reversibly methyl esterified on Leu-309 by leucine carboxyl methyltransferase 1 (LCMT1) and protein phosphatase methylesterase 1 (PPME1). Carboxyl methylation influences the affinity of the catalytic subunit for the different regulatory subunits, thereby modulating the PP2A holoenzyme's substrate specificity, enzyme activity and cellular localization.; Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.; May be monoubiquitinated by NOSIP.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'.