PON2 Recombinant Rabbit Monoclonal Antibody [JG35-80] (ET7108-19)
Catalog# ET7108-19
Rabbit Monoclonal to PON2
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WB
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IHC-P
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IP
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Human
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Mouse
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Rat
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-PON2 KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 39 kDa
Observed band size: 39,42 kDa
Exposure time: 40 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET7108-19) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
PON2 Recombinant Rabbit Monoclonal Antibody [JG35-80] (ET7108-19)
Immunogen
Recombinant protein within c-terminal human pon2 aa 160-354 / 354.
Host
Rabbit
Positive Control
A549 cell lysate, HCT116 cell lysate, human colon carcinoma tissue, human appendix tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JG35-80
RRID
APPLICATION DILUTION
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WB
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1:1.000-1:2,000
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IP
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1:10-1:50
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IHC-P
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1:50-1:200
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 39 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PON2
SYNONYMS
A esterase 2 antibody;A-esterase 2 antibody;Aromatic esterase 2 antibody;Paraoxonase 2 antibody;Paraoxonase nirs antibody;PON 2 antibody;PON2 antibody;PON2_HUMAN antibody;Serum aryldialkylphosphatase 2 antibody;Serum paraoxonase/arylesterase 2 antibody
SEQUENCE SIMILARITIES
Belongs to the paraoxonase family.
TISSUE SPECIFICITY
Widely expressed with highest expression in liver, lung, placenta, testis and heart.
POST-TRANSLATIONAL MODIFICATION
The signal sequence is not cleaved.
SUBCELLULAR LOCATION
Membrane.
FUNCTION
Paroxon is an organophosphorus anticholinesterase compound, used topically in the treatment of glaucoma. It is produced in vivo in mammals by microsomal oxidation of the insecticide parathion. Parathion is inert until transformed to paroxon. Paroxonase (paraoxonase or PON) is an arylesterase that is capable of hydrolyzing paroxon to produce p-nitrophenol. PONs are nonspecific and their classification is based not only on substrate specificity but also on tissue distribution, inhibition properties, and physicochemical characteristics such as electrophoretic mobility and molecular weight. In contrast to PON1, which is expressed mainly in the liver, PON2 is expressed in a variety of mouse tissues, including the pancreas. PON3 is associated with the high density lipoprotein fraction of serum. The genes which encode PON1-3 are physically linked and map to human chromosome 7q21.3.
CITATIONS
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Zhu, D., Zhang, M., He, B., Wan, Y., Wang, L., & Gao, F.
The role of sex and ovarian hormones in hippocampal damage and cognitive deficits induced by chronic exposure to hypobaric hypoxia
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WB
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Rat
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Citation
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