PLK1 Rabbit Polyclonal Antibody (HA500281)
Catalog# HA500281
Rabbit Polyclonal to PLK1
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WB
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FC
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Human
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Mouse
Western blot analysis of PLK1 on different lysates with Rabbit anti-PLK1 antibody (HA500281) at 1/1,000 dilution.
Lane 1: HeLa whole cell lysate
Lane 2: HeLa treated with 100nM Nocodazole for 24 hours whole cell lysate
Lane 3: HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate
Lane 4: HT-29 whole cell lysate
Lane 5: HT-29 treated with 100nM Nocodazole for 24 hours whole cell lysate
Lane 6: HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 68 kDa
Observed band size: 68 kDa
Exposure time: 2 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA500281) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:100,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Rabbit polyclonal primary
Product Name
PLK1 Rabbit Polyclonal Antibody (HA500281)
Immunogen
Synthetic peptide within human plk1 aa 1-50 / 603.
Host
Rabbit
Positive Control
HeLa whole cell lysate, HeLa treated with 100nM Nocodazole for 24 hours whole cell lysate, HeLa treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate, HT-29 whole cell lysate, HT-29 treated with 100nM Nocodazole for 24 hours whole cell lysate, HT-29 treated with 2mM thymidine for 16 hours then treated with 100nM Nocodazole for 24 hours whole cell lysate, HepG2 cell lysate, Hela cell lysate, mouse testis tissue lysate, Hela.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:1,000
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FC
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1:500-1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 68 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PLK1
SYNONYMS
Cell cycle regulated protein kinase antibody;PLK 1 antibody;PLK antibody;PLK-1 antibody;plk1 antibody;PLK1_HUMAN antibody;Polo like kinase 1 antibody;Polo-like kinase 1 antibody;Serine/threonine protein kinase 13 antibody;Serine/threonine protein kinase PLK1 antibody;Serine/threonine-protein kinase 13 antibody;Serine/threonine-protein kinase PLK1 antibody;STPK 13 antibody;STPK13 antibody
SUBCELLULAR LOCATION
Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Nucleus.
FUNCTION
Serine/threonine-protein kinase that performs several important functions throughout M phase of the cell cycle, including the regulation of centrosome maturation and spindle assembly, the removal of cohesins from chromosome arms, the inactivation of anaphase-promoting complex/cyclosome (APC/C) inhibitors, and the regulation of mitotic exit and cytokinesis. Polo-like kinase proteins acts by binding and phosphorylating proteins are that already phosphorylated on a specific motif recognized by the POLO box domains. PLK1 is high on non-attached kinetochores suggesting a role of PLK1 in kinetochore attachment or in spindle assembly checkpoint (SAC) regulation. Required for kinetochore localization of BUB1B. Regulates the dissociation of cohesin from chromosomes by phosphorylating cohesin subunits such as STAG2/SA2.
CITATIONS
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Downregulation of a CT10 regulator of kinase (Crk) promotes the formation of diapause embryos in the brine shrimp Artemia
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WB
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Brine shrimp Artemia
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Citation
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