Applications
-
WB
-
IHC-P
-
ICC
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Mouse monoclonal primary
Product Name
PLGF Mouse Monoclonal Antibody [3G3] (EM1701-88)
Immunogen
Recombinant protein within human plgf 1-150 aa.
Host
Mouse
Positive Control
JAR, LOVO, rat kidney tissue, human placenta tissue, mouse testis tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
3G3
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
2 ug/ul
PURIFICATION
Protein affinity purified.
MOLECULAR WEIGHT
25 kDa
Isotype
IgG1
APPLICATION DILUTION
-
WB
-
1:500
-
ICC
-
1:50
-
IHC-P
-
1:50-1:200
TARGET
UNIPROT #
PROTEIN NAME
PLGF
SYNONYMS
D12S1900 antibody; Pgf antibody; PGFL antibody; PIGF antibody; Placenta growth factor antibody; Placental growth factor antibody; Placental growth factor, vascular endothelial growth factor related protein antibody; PlGF 2 antibody; PlGF antibody; PLGF_HUMAN antibody; PlGF2 antibody; SHGC 10760 antibody
SEQUENCE SIMILARITIES
Belongs to the PDGF/VEGF growth factor family.
TISSUE SPECIFICITY
While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas.
POST-TRANSLATIONAL MODIFICATION
N-glycosylated.
SUBCELLULAR LOCATION
Secreted.
FUNCTION
The onset of angiogenesis is believed to be an early event in tumorigenesis and may facilitate tumor progression and metastasis. Several growth factors with angiogenic activity have been described. These include fibroblast growth factor (FGF), platelet derived growth factor (PDGF), vascular endothelial growth factor (VEGF) and placenta growth factor (PlGF). Like VEGF, several PlGF variants have been shown to arise from alternative mRNA splicings. Evidence has suggested VEGF to be an obligatory component in PlGF signaling. While VEGF homodimers and VEGF/PlGF heterodimers function as potent mediators of mitogenic and chemotactic responses in endothelial cells, PlGF homodimers are effectual only at extremely high concentrations. Indeed, many of the physiological effects attributed to VEGF may actually be a result of VEGF/PlGF. VEGF and PlGF share a common receptor, Flt-1, and may also activate Flk-1/KDR.