Plexin A1 Recombinant Rabbit Monoclonal Antibody [JU50-37] (ET1706-17)
Catalog# ET1706-17
Rabbit Monoclonal to Plexin A1
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WB
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Human
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Mouse
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Rat
Lane 1: K-562 cell lysate (20 µg/Lane)
Lane 2: HUVEC cell lysate (20 µg/Lane)
Lane 3: Mouse brain tissue lysate (40 µg/Lane)
Lane 4: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 211 kDa
Observed band size: 250 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1706-17) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Plexin A1 Recombinant Rabbit Monoclonal Antibody [JU50-37] (ET1706-17)
Immunogen
Recombinant protein within human plexin a1 aa 100-200 / 1,896.
Host
Rabbit
Positive Control
K-562 cell lysate, HUVEC cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JU50-37
RRID
APPLICATION DILUTION
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WB
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1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 211 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Plexin A1
SYNONYMS
NOV antibody;NOV/plexin A1 protein antibody;NOVP antibody;Plexin-A1 antibody;Plexin1 antibody;PlexinA1 antibody;PLXA1_HUMAN antibody;PLXN1 antibody;Plxna1 antibody;PLXNA1 protein antibody;Semaphorin receptor NOV antibody
SEQUENCE SIMILARITIES
Belongs to the plexin family.
TISSUE SPECIFICITY
Detected in fetal brain, lung, liver and kidney.
SUBCELLULAR LOCATION
Cell Membrane.
FUNCTION
Coreceptor for SEMA3A, SEMA3C, SEMA3F and SEMA6D. Necessary for signaling by class 3 semaphorins and subsequent remodeling of the cytoskeleton. Plays a role in axon guidance, invasive growth and cell migration. Class 3 semaphorins bind to a complex composed of a neuropilin and a plexin. The plexin modulates the affinity of the complex for specific semaphorins, and its cytoplasmic domain is required for the activation of down-stream signaling events in the cytoplasm.
CITATIONS
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Liu, Y., Hao, Y., Zhao, H., Zhang, Y., Cheng, D., Zhao, L., Peng, Y., Lu, Y., & Li, Y.
PlexinA1 activation induced by β2-AR promotes epithelial-mesenchymal transition through JAK-STAT3 signaling in human gastric cancer cells
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WB
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IF
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Human
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Citation
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