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PRODUCT CODE: EM1701-48

PLEKHH1 Mouse Monoclonal Antibody [3-E11] (EM1701-48)

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

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Western blot analysis of PLEKHH1 on different cell lysates using anti-PLEKHH1 antibody at 1/100 dilution.<br /> Positive control:<br /> Lane 1: MCF-7 <br /> Lane 2: A431<br /> Lane 3: SH-SY5Y
  • Western blot analysis of PLEKHH1 on different cell lysates using anti-PLEKHH1 antibody at 1/100 dilution.<br /> Positive control:<br /> Lane 1: MCF-7 <br /> Lane 2: A431<br /> Lane 3: SH-SY5Y
  • ICC staining PLEKHH1 (green) in A431 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PLEKHH1 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PLEKHH1 antibody. Counter stained with hematoxylin.
Western blot analysis of PLEKHH1 on different cell lysates using anti-PLEKHH1 antibody at 1/100 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: A431
Lane 3: SH-SY5Y

Applications

  • WB

  • IHC-P

  • ICC

REACTIVITY

  • Human

  • Mouse

SPECIFICATIONS

Product Type

Mouse monoclonal primary

Product Name

PLEKHH1 Mouse Monoclonal Antibody [3-E11] (EM1701-48)

Immunogen

Recombinant protein within human plekhh1 aa 1,059-1,364 / 1,364.

Host

Mouse

Positive Control

MCF-7, A431, SH-SY5Y, human kidney tissue, mouse kidney tissue.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

3-E11

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

2 ug/ul

PURIFICATION

Protein G purified.

MOLECULAR WEIGHT

151/30 kDa

Isotype

IgG2b

APPLICATION DILUTION

  • WB

  • 1:500

  • ICC

  • 1:50

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

Q9ULM0

PROTEIN NAME

PLEKHH1

SYNONYMS

KIAA1200 antibody; PH domain containing family H member 1 antibody; Pleckstrin homology domain containing, family H (with MyTH4 domain) member 1 antibody; pleckstrin homology domain-containing family H member 1 antibody

SUBCELLULAR LOCATION

Cytoskeleton.

FUNCTION

PLEKHH1 (Pleckstrin Homology, MyTH4 And FERM Domain Containing H1) is a Protein Coding gene. Diseases associated with PLEKHH1 include Gestational Trophoblastic Neoplasm. Critical component of the guidance pathway underlying endothelial cell migration and blood vessel patterning. Involved in mediating membrane localization of ephrin proteins, which have been shown to provide guidance cues for endothelial cell migration.