Applications
-
WB
-
IHC-P
-
IP
REACTIVITY
-
Human
-
Mouse
-
Rat
SPECIFICATIONS
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Plectin Recombinant Rabbit Monoclonal Antibody [SY29-04] (ET1607-80)
Immunogen
Recombinant protein
Host
Rabbit
Positive Control
Rat skeletal muscle tissue, mouse skeletal muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY29-04
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 ug/ul
PURIFICATION
Protein A purified.
MOLECULAR WEIGHT
531 kDa
Isotype
IgG
APPLICATION DILUTION
-
WB
-
1:1,000
-
IHC-P
-
1:100-1:500
TARGET
UNIPROT #
PROTEIN NAME
Plectin
SYNONYMS
EBS1 antibody; EBSO antibody; HD1 antibody; Hemidesmosomal protein 1 antibody; PCN antibody; pleC antibody; PLEC_HUMAN antibody; PLEC1 antibody; PLEC1b antibody; Plectin 1 antibody; Plectin 1 intermediate filament binding protein 500kDa antibody; Plectin 6 antibody; Plectin antibody; Plectin-1 antibody; PLTN antibody
SEQUENCE SIMILARITIES
Belongs to the plakin or cytolinker family.
TISSUE SPECIFICITY
Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
SUBCELLULAR LOCATION
Cytoplasm, Cell junction.
FUNCTION
Plectin is an abundant cytoskeletal protein that is involved in cytoplasm stabilization. Plectin has been shown to crosslink intermediate filaments to microtubules and microfilaments, and to anchor intermediate filaments to the plasma and nuclear membranes. Plectin binds both Lamin B and vimentin, and this binding is regulated by a variety of protein kinases. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions. Plectin is also a substrate for p34cdc2 kinase. Several alternative splice isoforms of plectin are known to exist. Mutations in human plectin are known to cause epidermolysis bullosa simplex with muscular dystrophy (EBS-MD).