Plectin Recombinant Rabbit Monoclonal Antibody [SY29-04] (ET1607-80)
Catalog# ET1607-80
Rabbit Monoclonal to Plectin
-
WB
-
IHC-P
-
IP
-
Human
-
Mouse
-
Rat
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1607-80) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Plectin Recombinant Rabbit Monoclonal Antibody [SY29-04] (ET1607-80)
Immunogen
Recombinant protein within human plectin aa 2-101 / 4,684.
Host
Rabbit
Positive Control
Rat skeletal muscle tissue, mouse skeletal muscle tissue, human striated muscle tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
SY29-04
APPLICATION DILUTION
-
WB
-
1:1,000
-
IHC-P
-
1:50-1:500
-
IP
-
Use at an assay dependent concentration.
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 532 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Plectin
SYNONYMS
EBS1 antibody;EBSO antibody;HD1 antibody;Hemidesmosomal protein 1 antibody;PCN antibody;pleC antibody;PLEC_HUMAN antibody;PLEC1 antibody;PLEC1b antibody;Plectin 1 antibody;Plectin 1 intermediate filament binding protein 500kDa antibody;Plectin 6 antibody;Plectin antibody;Plectin-1 antibody;PLTN antibody
SEQUENCE SIMILARITIES
Belongs to the plakin or cytolinker family.
TISSUE SPECIFICITY
Widely expressed with highest levels in muscle, heart, placenta and spinal cord.
POST-TRANSLATIONAL MODIFICATION
Phosphorylated by CDK1; regulates dissociation from intermediate filaments during mitosis.
SUBCELLULAR LOCATION
Cell junction, Cytoplasm, Cytoskeleton.
FUNCTION
Plectin is a giant protein found in nearly all mammalian cells which acts as a link between the three main components of the cytoskeleton: actin microfilaments, microtubules and intermediate filaments. Plectin links the cytoskeleton to junctions found in the plasma membrane that structurally connect different cells. By holding these different networks together, plectin plays an important role in maintaining the mechanical integrity and viscoelastic properties of tissues. Plectin binds both Lamin B and vimentin, and this binding is regulated by a variety of protein kinases. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions.
CITATIONS
-
Gineste, C., Youhanna, S., Vorrink, S. U., Henriksson, S., Hernández, A., Cheng, A. J., Chaillou, T., Buttgereit, A., Schneidereit, D., Friedrich, O., Hultenby, K., Bruton, J. D., Ivarsson, N., Sandblad, L., Lauschke, V. M., & Westerblad, H.
Enzymatically dissociated muscle fibers display rapid dedifferentiation and impaired mitochondrial calcium control
-
IF
-
Mouse
-
Citation
-
Popup
Use this popup to embed a mailing list sign up form. Alternatively use it as a simple call to action with a link to a product or a page.
Age verification
By clicking enter you are verifying that you are old enough to consume alcohol.