PRODUCT CODE: ER2001-67

Pleckstrin Rabbit Polyclonal Antibody (ER2001-67)

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

Western blot analysis of Pleckstrin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-67, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: U937 cell lysate<br />
Lane 2: Rat spleen tissue lysate<br />
Lane 2: Mouse brain tissue lysate
  • Western blot analysis of Pleckstrin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-67, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.<br />
Positive control: <br />
Lane 1: U937 cell lysate<br />
Lane 2: Rat spleen tissue lysate<br />
Lane 2: Mouse brain tissue lysate
Western blot analysis of Pleckstrin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER2001-67, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: U937 cell lysate
Lane 2: Rat spleen tissue lysate
Lane 2: Mouse brain tissue lysate

Applications

  • WB

REACTIVITY

  • Human

  • Mouse

  • Rat

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

Pleckstrin Rabbit Polyclonal Antibody (ER2001-67)

Immunogen

Synthetic peptide within human pleckstrin aa 300-350.

Host

Rabbit

Positive Control

U937 cell lysate, rat spleen tissue lysate, mouse brain tissue lysate.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

40 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

TARGET

UNIPROT #

PROTEIN NAME

Pleckstrin

SYNONYMS

FLJ27168 antibody; OTTHUMP00000159962 antibody; p47 antibody; Platelet 47 kDa protein antibody; Platelet and leukocyte C kinase substrate antibody; Platelet P47 antibody; Platelet p47 protein antibody; Pleckstrin antibody; PLEK antibody; PLEK_HUMAN antibody

SUBCELLULAR LOCATION

Cytosol, extracellular region, plasma membrane, ruffle membrane, cytoplasm, membrane.

FUNCTION

Major protein kinase C substrate of platelets.Pleckstrin is a protein found in platelets. The name derives from platelet and leukocyte C kinase substrate and the KSTR string of amino acids.