PRODUCT CODE: R1512-4

PLCG2 Rabbit Polyclonal Antibody (R1512-4)

Applications

  • WB

  • ICC

  • IHC

  • FC

REACTIVITY

  • Human

Western blot analysis of PLCG2 on Raji cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1512-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • Western blot analysis of PLCG2 on Raji cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1512-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
  • ICC staining of PLCG2 in AGS cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (R1512-4, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PLCG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (R1512-4, 1/400) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
  • Flow cytometric analysis of PLCG2 was done on Daudi cells. The cells were fixed, permeabilized and stained with the primary antibody (R1512-4, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Western blot analysis of PLCG2 on Raji cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (R1512-4, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Applications

  • WB

  • ICC

  • IHC

  • FC

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PLCG2 Rabbit Polyclonal Antibody (R1512-4)

Immunogen

Synthetic peptide within human plcg2 aa 1200-1265.

Host

Rabbit

Positive Control

Raji cell lysates, AGS, human tonsil tissue, Daudi.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4Á¾ after thawing. Aliquot store at -20Á¾. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

148 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:50-1:100

  • IHC

  • 1:100-1:400

  • FC

  • 1:50-1:100

TARGET

UNIPROT #

PROTEIN NAME

PLCG2

SYNONYMS

1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase gamma 2 antibody; 1-phosphatidylinositol-4 antibody; 5-bisphosphate phosphodiesterase gamma-2 antibody; EC 3.1.4.11 antibody; Phosphoinositide phospholipase C antibody; Phosphoinositide phospholipase C-gamma-2 antibody; Phospholipase C gamma 2 antibody; Phospholipase C, gamma 2 (phosphatidylinositol specific) antibody; Phospholipase C-gamma-2 antibody; Phospholipase C-IV antibody; PLC 2 antibody; PLC gamma 2 antibody; PLC IV antibody; PLC-gamma-2 antibody; PLC-IV antibody; Plcg2 antibody; PLCG2_HUMAN antibody

SUBCELLULAR LOCATION

Cytosol, Autolysosome.

FUNCTION

Phosphoinositide-specific phospholipase C (PLC) plays a critical role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate.There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC e. After stimulation of the collagen receptor glycoprotein VI in human platelets, PLC γ2 associates with several tyrosine-phosphorylated proteins (Syk, SLP-76, Lyn, linker for activation of T cells (LAT) and the FcR γ chain), which bind to its C-terminal SH2 domain. PLC γ1 associates with Syk in B cells, but PLC γ2 does not associate with Syk in platelets. The C-terminal SH2 domain is involved in the regulation of PLC γ2. In addition, Btk can induce PLC γ2 tyrosine phosphorylation and initiate calcium moblization in CD72-stimulated B lymphocytes.