Placental alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [JM22-53] (ET1705-21)
Catalog# ET1705-21
Rabbit Monoclonal to Placental alkaline phosphatase
-
WB
-
IF-Tissue
-
IHC-P
-
Human
Lane 1: A431 cell lysate
Lane 2: SiHa cell lysate
Lane 3: AGS cell lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 58 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 14 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ET1705-21) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
Placental alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [JM22-53] (ET1705-21)
Immunogen
Synthetic peptide within human placental alkaline phosphatase aa 171-220 / 535.
Host
Rabbit
Positive Control
A431 cell lysate, SiHa cell lysate, AGS cell lysate, human placenta tissue lysates, human placenta tissue, human seminoma tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JM22-53
RRID
APPLICATION DILUTION
-
WB
-
1:500-1:5,000
-
IF-Tissue
-
1:500
-
IHC-P
-
1:50-1:400
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 58 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
Placental alkaline phosphatase
SYNONYMS
Alkaline phosphatase antibody;Alkaline phosphatase placental antibody;Alkaline phosphatase placental type antibody;Alkaline phosphatase Regan isozyme antibody;ALP antibody;Alp1 antibody;ALPP antibody;FLJ61142 antibody;Germ-cell alkaline phosphatase antibody;nagao isozyme antibody;OTTHUMP00000164354 antibody;PALP antibody;Placental alkaline phosphatase 1 antibody;placental heat-stable alkaline phosphatase antibody;placental type antibody;PLAP antibody;PLAP-1 antibody;PLAP1 antibody;PPB1_HUMAN antibody
SEQUENCE SIMILARITIES
Belongs to the alkaline phosphatase family.
TISSUE SPECIFICITY
Detected in placenta (at protein level).
SUBCELLULAR LOCATION
Cell membrane.
FUNCTION
Alkaline phosphatase, placental type also known as placental alkaline phosphatase (PLAP) is an allosteric enzyme that in humans is encoded by the ALPP gene. There are at least four distinct but related alkaline phosphatases: intestinal (ALPI), placental (this enzyme), placental-like (ALPPL2), and liver/bone/kidney (ALPL) (tissue-nonspecific). The first three are located together on chromosome 2, whereas the tissue-nonspecific form is located on chromosome 1. The coding sequence for this form of alkaline phosphatase is unique in that the 3' untranslated region contains multiple copies of an Alu family repeat. In addition, this gene is polymorphic and three common alleles (type 1, type 2, and type 3) for this form of alkaline phosphatase have been well-characterized. Alkaline phosphatase, placental type is a membrane-bound glycosylated dimeric enzyme, also referred to as the heat-stable form, that is expressed primarily in the placenta, although it is closely related to the intestinal form of the enzyme as well as to the placental-like form.
