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PRODUCT CODE: ET1705-21

Placental alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [JM22-53] (ET1705-21)

  • Recombinant

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

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Western blot analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution.
  • Western blot analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution.
  • ICC staining PLAP in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PLAP in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-PLAP antibody. Counter stained with hematoxylin.
Western blot analysis of PLAP on human placenta tissue (1) and Hela cell (2) lysate using anti-PLAP antibody at 1/1,000 dilution.

Applications

  • WB

  • ICC

  • IF

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Recombinant Rabbit monoclonal primary

Product Name

Placental alkaline phosphatase Recombinant Rabbit Monoclonal Antibody [JM22-53] (ET1705-21)

Immunogen

Recombinant protein

Host

Rabbit

Positive Control

Human placenta tissue, Hela.

Conjugation

Unconjugated

Clonality

Monoclonal

Clone Number

JM22-53

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C or -80C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Protein A purified.

MOLECULAR WEIGHT

70 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:2,000

  • ICC/IF

  • 1:50-1:200

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

P05187

PROTEIN NAME

Placental alkaline phosphatase

SYNONYMS

Alkaline phosphatase antibody; Alkaline phosphatase placental antibody; Alkaline phosphatase placental type antibody; Alkaline phosphatase Regan isozyme antibody; ALP antibody; Alp1 antibody; ALPP antibody; FLJ61142 antibody; Germ-cell alkaline phosphatase antibody; nagao isozyme antibody; OTTHUMP00000164354 antibody; PALP antibody; Placental alkaline phosphatase 1 antibody; placental heat-stable alkaline phosphatase antibody; placental type antibody; PLAP antibody; PLAP-1 antibody; PLAP1 antibody; PPB1_HUMAN antibody

SEQUENCE SIMILARITIES

Belongs to the alkaline phosphatase family.

TISSUE SPECIFICITY

Detected in placenta (at protein level).

SUBCELLULAR LOCATION

Cell membrane.

FUNCTION

Alkaline phosphatases (AP) are glycosyl-phosphatidylinositol (GPI)-anchored, dimeric, Zn2+-metallated glycoproteins that catalyze the hydrolysis of phosphomonoesters into an inorganic phosphate and an alcohol. Placental alkaline phosphatase (also known as PLAP, ALPP, PALP, placental ALP-1 or Regan isozyme) is a 530 amino acid, tissue-specific AP that is expressed in the placenta, the serum of pregnant women and ectopically expressed in various cancers, including those of the ovary and testis. PLAP may assist in guiding migratory cells and transporting specific molecules, such as fatty acids and immunoglobulins, across the plasma membrane. The three tissue-specific APs identified in human, PLAP, germ cell AP (GCAP) and intestinal AP, are 90-98% homologous and their genes are clustered on chromosome 2q.