PRODUCT CODE: ER1802-70

PKM2 Rabbit Polyclonal Antibody (ER1802-70)

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

Western blot analysis of PKM2 on 293 cell lysate using anti-PKM2 antibody at 1/500 dilution.
  • Western blot analysis of PKM2 on 293 cell lysate using anti-PKM2 antibody at 1/500 dilution.
  • ICC staining PKM2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PKM2 in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • ICC staining PKM2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human thyroid gland cancer tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-PKM2 antibody. Counter stained with hematoxylin.
Western blot analysis of PKM2 on 293 cell lysate using anti-PKM2 antibody at 1/500 dilution.

Applications

  • WB

  • ICC

  • IHC-P

REACTIVITY

  • Human

SPECIFICATIONS

Product Type

Rabbit polyclonal primary

Product Name

PKM2 Rabbit Polyclonal Antibody (ER1802-70)

Immunogen

Synthetic peptide within human pkm2 aa 370-450.

Host

Rabbit

Positive Control

293, A549, F9, PC-3M, human tonsil tissue, human thyroid gland cancer tissue, human colon cancer tissue, human breast cancer tissue.

Conjugation

Unconjugated

Clonality

Polyclonal

PROPERTIES

Form

Liquid

Storage Condition

Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.

Storage Buffer

1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 ug/ul

PURIFICATION

Peptide affinity purified.

MOLECULAR WEIGHT

58 kDa

Isotype

IgG

APPLICATION DILUTION

  • WB

  • 1:500-1:1,000

  • ICC

  • 1:100-1:500

  • IHC-P

  • 1:50-1:200

TARGET

UNIPROT #

PROTEIN NAME

PKM2

SYNONYMS

CTHBP antibody; Cytosolic thyroid hormone binding protein antibody; Cytosolic thyroid hormone-binding protein antibody; KPYM_HUMAN antibody; MGC3932 antibody; OIP 3 antibody; OIP-3 antibody; OIP3 antibody; OPA interacting protein 3 antibody; Opa-interacting protein 3 antibody; p58 antibody; PK muscle type antibody; PK, muscle type antibody; PK2 antibody; PK3 antibody; PKM antibody; PKM2 antibody; pykm antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase 3 antibody; Pyruvate kinase isozymes M1/M2 antibody; Pyruvate kinase muscle antibody; Pyruvate kinase muscle isozyme antibody; pyruvate kinase PKM antibody; Pyruvate kinase, muscle 2 antibody; TCB antibody; THBP1 antibody; Thyroid hormone binding protein 1 antibody; Thyroid hormone binding protein cytosolic antibody; Thyroid hormone-binding protein 1 antibody; Tumor M2 PK antibody; Tumor M2-PK antibody

SEQUENCE SIMILARITIES

Belongs to the pyruvate kinase family.

TISSUE SPECIFICITY

Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.

POST-TRANSLATIONAL MODIFICATION

ISGylated.; Under hypoxia, hydroxylated by EGLN3.; Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.; FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.

SUBCELLULAR LOCATION

Cytoplasm. Nucleus.

FUNCTION

In mammals, four different isoenzymes exist for pyruvate kinase. Pyruvate kinases are responsible for catalyzing the final step in glycolysis: the conversion of phosphoenolpyruvate to pyruvate with the coinciding generation of ATP. The PKM (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events. Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1,6-bisphosphate. In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1. The M2-type isoform also interacts with Oct-4 via its C-terminal domain, functioning to enhance Oct-4 transcriptional activity. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.