PKM2 Rabbit Polyclonal Antibody (ER1802-70)
Catalog# ER1802-70
Rabbit Polyclonal to PKM2
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WB
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IF-Cell
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IHC-P
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Human
Lane 1: Wild-type MDA-MB-231 whole cell lysate.
Lane 2: PKM knockout MDA-MB-231 whole cell lysate.
ER1802-70 was shown to specifically react with PKM in wild-type MDA-MB-231 cells. No band was observed when PKM knockout samples were tested. Wild-type and PKM knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-PKM antibody (ER1802-70, 1/500) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China).
Specifications
Product Type
Rabbit polyclonal primary
Product Name
PKM2 Rabbit Polyclonal Antibody (ER1802-70)
Immunogen
Synthetic peptide within human pkm2 aa 370-450.
Host
Rabbit
Positive Control
Wild-type MDA-MB-231 whole cell lysate, SiHa cell lysate, A549 cell lysate, PC-3 cell lysate, A549, F9, PC-3M, human tonsil tissue, human thyroid carcinoma tissue, human colon cancer tissue, human breast cancer tissue.
Conjugation
Unconjugated
Clonality
Polyclonal
RRID
APPLICATION DILUTION
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WB
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1:500-1:1,000
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IF-Cell
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1:100-1:500
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IHC-P
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1:50-1:400
PROPERTIES
Form
Liquid
Storage Condition
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
Storage Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Immunogen affinity purified.
Molecular Weight
Predicted band size: 58 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
PKM2
SYNONYMS
CTHBP antibody;Cytosolic thyroid hormone binding protein antibody;Cytosolic thyroid hormone-binding protein antibody;KPYM_HUMAN antibody;MGC3932 antibody;OIP 3 antibody;OIP-3 antibody;OIP3 antibody;OPA interacting protein 3 antibody;Opa-interacting protein 3 antibody;p58 antibody;PK muscle type antibody;PK, muscle type antibody;PK2 antibody;PK3 antibody;PKM antibody;PKM2 antibody;pykm antibody;Pyruvate kinase 2/3 antibody;Pyruvate kinase 3 antibody;Pyruvate kinase isozymes M1/M2 antibody;Pyruvate kinase muscle antibody;Pyruvate kinase muscle isozyme antibody;pyruvate kinase PKM antibody;Pyruvate kinase, muscle 2 antibody;TCB antibody;THBP1 antibody;Thyroid hormone binding protein 1 antibody;Thyroid hormone binding protein cytosolic antibody;Thyroid hormone-binding protein 1 antibody;Tumor M2 PK antibody;Tumor M2-PK antibody
SEQUENCE SIMILARITIES
Belongs to the pyruvate kinase family.
TISSUE SPECIFICITY
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
POST-TRANSLATIONAL MODIFICATION
ISGylated.; Under hypoxia, hydroxylated by EGLN3.; Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.; FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
SUBCELLULAR LOCATION
Cytoplasm. Nucleus.
FUNCTION
In mammals, four different isoenzymes exist for pyruvate kinase. Pyruvate kinases are responsible for catalyzing the final step in glycolysis: the conversion of phosphoenolpyruvate to pyruvate with the coinciding generation of ATP. The PKM (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events. Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1,6-bisphosphate. In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1. The M2-type isoform also interacts with Oct-4 via its C-terminal domain, functioning to enhance Oct-4 transcriptional activity. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.
CITATIONS
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Yangfan Liu , Yu Sun , Jin Yang ,et al
DNMT1-targeting remodeling global DNA hypomethylation for enhanced tumor suppression and circumvented toxicity in oral squamous cell carcinoma
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IHC
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Human
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Citation
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