Rabbit polyclonal primary
PKM2 Rabbit Polyclonal Antibody (ER1802-70)
Synthetic peptide within human pkm2 aa 370-450.
293, A549, F9, PC-3M, human tonsil tissue, human thyroid gland cancer tissue, human colon cancer tissue, human breast cancer tissue.
Store at +4C after thawing. Aliquot store at -20C. Avoid repeated freeze / thaw cycles.
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Peptide affinity purified.
CTHBP antibody; Cytosolic thyroid hormone binding protein antibody; Cytosolic thyroid hormone-binding protein antibody; KPYM_HUMAN antibody; MGC3932 antibody; OIP 3 antibody; OIP-3 antibody; OIP3 antibody; OPA interacting protein 3 antibody; Opa-interacting protein 3 antibody; p58 antibody; PK muscle type antibody; PK, muscle type antibody; PK2 antibody; PK3 antibody; PKM antibody; PKM2 antibody; pykm antibody; Pyruvate kinase 2/3 antibody; Pyruvate kinase 3 antibody; Pyruvate kinase isozymes M1/M2 antibody; Pyruvate kinase muscle antibody; Pyruvate kinase muscle isozyme antibody; pyruvate kinase PKM antibody; Pyruvate kinase, muscle 2 antibody; TCB antibody; THBP1 antibody; Thyroid hormone binding protein 1 antibody; Thyroid hormone binding protein cytosolic antibody; Thyroid hormone-binding protein 1 antibody; Tumor M2 PK antibody; Tumor M2-PK antibody
Belongs to the pyruvate kinase family.
Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells.
ISGylated.; Under hypoxia, hydroxylated by EGLN3.; Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy.; FGFR1-dependent tyrosine phosphorylation is reduced by interaction with TRIM35.
In mammals, four different isoenzymes exist for pyruvate kinase. Pyruvate kinases are responsible for catalyzing the final step in glycolysis: the conversion of phosphoenolpyruvate to pyruvate with the coinciding generation of ATP. The PKM (pyruvate kinase, muscle) gene encodes the M1- and M2-type isoenzymes through alternative splicing events. Both M1- and M2-type isoforms exists as tetramers and are stimulated by fructose 1,6-bisphosphate. In addition, both isoforms exhibit thyroid hormone binding activity and may be referred to as CTHBP (cytosolic thyroid hormone-binding protein) or THBP1. The M2-type isoform also interacts with Oct-4 via its C-terminal domain, functioning to enhance Oct-4 transcriptional activity. Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity.